Recombinant Human C1QB Protein (Baculovirus-Insect, His Tag)

Biological Activity

Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. The recombinant human C1QB consists of 237 amino acids with the predicted molecular mass of 25.2 kDa. A DNA sequence encoding the human C1QB (NP_000482.3) precursor (Met 1-Ala 253) was expressed, with a carboxy-terminal polyhistidine tag.

Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, 0.5mM TCEP, pH 7.5.

Chemical Information

Storage

Stable for up to twelve months from date of receipt at -20℃ to -80℃

References

[1] Isabelle Bally, et al. J Immunol Methods. Functional recombinant human complement C1q with different affinity tags

[2] Hongyu Yuan, et al. Acta Crystallogr F Struct Biol Commun. Crystallization and preliminary crystallographic studies of the complement 1qA globular domain from zebrafish, Dare-C1qAgD

[3] Silvia Stojanov, et al. Proc Natl Acad Sci U S A. Periodic fever, aphthous stomatitis, pharyngitis, and adenitis (PFAPA) is a disorder of innate immunity and Th1 activation responsive to IL-1 blockade

[4] Yu-Lan Hu, et al. J Biol Chem. Characterization of C1q in teleosts: insight into the molecular and functional evolution of C1q family and classical pathway