Apolipoprotein E (ApoE) produced by the liver and circulating macrophages, that is a constituent of every plasma lipoprotein except the smallest low density lipoproteins (LDL). It is a key player in the recycling and redistribution of lipids and cholesterol. ApoE is a ligand with a high affinity for low density lipoprotein receptors (LDLR). It regulates the activity of enzymes that metabolize lipids and also makes lipids soluble. Mice and humans that lack ApoE cannot remove excess lipoproteins from the plasma and have an increased risk of atherosclerosis. Defective binding of ApoE to its receptors will lead to accumulation of cholesterolrich lipoprotein particles in the plasma; this is the cause of type III hyperlipoproteinemia. There are three main isoforms of ApoE all products of alleles at a single gene locus: E2 (Cys112, Cys158), E3 (C112, Arg158), and E4 (Arg112, Arg158). Inheritance of ApoE4 has been associated with earlier onset of Alzheimer’s disease, Parkinson’s disease and increased likelihood of dementia.

P02649 VAR_000652

GPKVEQAVET EPEPELRQQT EWQSGQRWEL ALGRFWDYLR WVQTLSEQVQ EELLSSQVTQ ELRALMDETM KELKAYKSEL EEQLTPVAEE TRARLSKELQ AAQARLGADM EDVRGRLVQY RGEVQAMLGQ STEELRVRLA SHLRKLRKRL LRDADDLQKR LAVYQAGARE GAERGLSAIR ERLGPLVEQG RVRAATVGSL AGQPLQERAQ AWGERLRARM EEMGSRTRDR LDEVKEQVAE VRAKLEEQAQ QIRLQAEAFQ ARLKSWFEPL VEDMQRQWAG LVEKVQAAVG TSAAPVPSDN H

Lyophilized

The molecular weight of Recombinant Human ApoE4 is 34.4 kDa.

The protein was 0.2 µm filtered and lyophilized from 20 Mm Sodium Phosphate, pH 7.8

The lyophilized protein should be stored desiccated at 20°C. The reconstituted protein can be store at 28°C for 1 week. For longterm storage of the reconstituted protein add a carrier protein such as 0.1% BSA, aliquot into working volumes and store at 20°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1.) Yang, Hong et al. PLoS One. 2012;7(9):e44430. 2012 Sep 11 PubMed