Rat Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit
Referência CSB-E13708r-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
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| Size | 96T,5×96T,10×96T |
Product Details
Associated names
N/A
Abbreviation
CK 18M30
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 mIU/mL-100 mIU/mL
Sensitivity
0.39 mIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 97 | |
| Range % | 91-102 | ||
| 1:2 | Average % | 94 | |
| Range % | 90-98 | ||
| 1:4 | Average % | 84 | |
| Range % | 80-88 | ||
| 1:8 | Average % | 103 | |
| Range % | 99-112 |
Recovery
| The recovery of rat CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat CK 18M30 ELISA Kit was designed for the quantitative measurement of Rat CK 18M30 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL .
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