Quimigen Portugal > Rat IgA (HC) Antibody - FITC Conjugated
Rat IgA (HC) Antibody - FITC Conjugated
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Rat IgA (HC) Antibody - FITC Conjugated (OASA04156)
| Datasheets/Manuals | Printable datasheet for Rat IgA (HC) Antibody - FITC Conjugated (OASA04156) |
|---|
| Predicted Species Reactivity | Rat |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | MARA-1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | FITC |
| Application | FC |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography Preservative Stabilisers: 0.09% - Sodium Azide 50% - Glycerol |
| :: | Approx Protein Conc: IgG concentration 1 mg/ml Buffer Solutions: Phosphate buffered saline |
| Reconstitution and Storage | -20°C |
| Immunogen | Purified IR1060 IgA rat myeloma protein. |
| Purification | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 1 mg/ml |
| Specificity | IgA |
| Application Info | Flow Cytometry: 5ug/ml |
| Reference | 1. Kohler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity Nature, 256: 495-497. 2. Schulman, M., Wilde, C.D. & Kohler, G. (1978) A better cell line for making hybridomas secreting specific antibodies Nature, 276: 269-270. 3. Bazin, H., Beckers, A. & Querinjean, P. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c Eur.J. Immunol. 4: 44-48. 4. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Platteau, B. & Urbain, J.J. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat Immunol 121: 2077-2082. 5. Bazin, H., et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum J. Immunol. Methods 71: 9-16. 6. Grabar and Williams (1953) Method permitting the combined study of the electrophoretic and the immunochemical properties of protein mixtures; application to blood serum. Biochem. Biophys. Acta 10: 193. |
|---|---|
| Protein Name | Ig alpha-1 chain C region |
| Description of Target | MOUSE ANTI RAT IgA HEAVY CHAIN:FITC |
| Uniprot ID | P01876 |
| Protein Size (# AA) | 131 |





