Rat IFN gamma ELISpot Kit
Referência MBS335702-1x96-PC
Tamanho : 1x96Wells(Pre-coated-plate)
Marca : MyBiosource
Contactar o distribuidor local :
Telefone : +1 850 650 7790
IFN Gamma ELISpot Kit for Rat
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335702Principle of the assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any… More details
Product Overview
Online Datasheet in PDFProduct Name
IFN gamma, ELISpot Kit
Full Product Name
Rat IFN gamma ELISpot Kit
Product Gene Name
IFN gamma elispot kit
Product Synonym Names
IFN-g
Species Reactivity
Rat. No cross reactivity with other rat cytokines
Molecular Weight
19,348 Da
OMIM
147570
Intended Uses
ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Product Categories/Family for IFN gamma elispot kit
Rat ELISpot Kit
Other Notes
Small volumes of IFN gamma elispot kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IFN gamma purchase
MBS335702 is an ELISpot kit targeting IFN gamma. Also known as Rat IFN gamma ELISpot Kit. Reactive in Rat. No cross reactivity with other rat cytokines.
Additional Details
NCBI/Uniprot data below describe general gene information for IFN gamma. It may not necessarily be applicable to this product.
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
UniProt Protein Name
Interferon gamma
UniProt Synonym Protein Names
Immune interferon
Protein Family
Interferon gamma receptor
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_HUMAN
NCBI Protein Information
interferon gamma
NCBI Summary for IFN gamma
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
UniProt Comments for IFN gamma
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
Protein type: Secreted; Membrane protein, integral; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To
Show more Protein type: Secreted; Membrane protein, integral; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with IFN gamma elispot kit
| Reference Product | PubMed Publications |
|---|---|
| TNF elispot kit | >181 publications with IFN gamma and TNF |
| IL2 elispot kit | >70 publications with IFN gamma and IL2 |
| TBX21 elispot kit | >61 publications with IFN gamma and TBX21 |
| STAT1 elispot kit | >48 publications with IFN gamma and STAT1 |
| TGFB1 elispot kit | >47 publications with IFN gamma and TGFB1 |
| IFNGR1 elispot kit | >32 publications with IFN gamma and IFNGR1 |
| IRF1 elispot kit | >19 publications with IFN gamma and IRF1 |
| IFNGR2 elispot kit | >17 publications with IFN gamma and IFNGR2 |
| IL18R1 elispot kit | >2 publications with IFN gamma and IL18R1 |
Pathways associated with IFN gamma elispot kit
| Products by Pathway | Pathway Diagram |
|---|---|
| ATF-2 Transcription Factor Network Pathway antibodies | ATF-2 Transcription Factor Network Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Amoebiasis Pathway antibodies | Amoebiasis Pathway Diagram |
| Amoebiasis Pathway antibodies | Amoebiasis Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
Diseases associated with IFN gamma elispot kit
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >173 publications with IFN gamma and Necrosis |
| Autoimmune Diseases Antibodies | >97 publications with IFN gamma and Autoimmune Diseases |
| Respiratory Tract Diseases Antibodies | >76 publications with IFN gamma and Respiratory Tract Diseases |
| Hepatitis Antibodies | >38 publications with IFN gamma and Hepatitis |
| Lymphoproliferative Disorders Antibodies | >23 publications with IFN gamma and Lymphoproliferative Disorders |
| HIV Infections Antibodies | >21 publications with IFN gamma and HIV Infections |
| Colitis Antibodies | >18 publications with IFN gamma and Colitis |
| Hepatitis C Antibodies | >18 publications with IFN gamma and Hepatitis C |
| Diabetes Mellitus, Type 1 Antibodies | >18 publications with IFN gamma and Diabetes Mellitus, Type 1 |
| Arthritis, Rheumatoid Antibodies | >16 publications with IFN gamma and Arthritis, Rheumatoid |
Organs/Tissues associated with IFN gamma elispot kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >561 publications with IFN gamma and Blood |
| Kidney Antibodies | >47 publications with IFN gamma and Kidney |
| Lymph Node Antibodies | >29 publications with IFN gamma and Lymph Node |