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Rat IFN-gamma ELISA Kit
Rat IFN-gamma ELISA Kit
RayBio® Rat IFN-gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 100 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 100 pg/ml - 30000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01581 |
| Gene Id | 25712 |
| Gene Symbols | IFNG |
| Protein Name / Synonyms | Interferon gamma (IFN-gamma) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Autophagy, Innate Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Rat IFN-gamma qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat IFN-gamma ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IFN-gamma (IFNG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Immuno-antioxidative reno-modulatory effectiveness of Echinacea purpurea extract against bifenthrin-induced renal poisoning
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (renal tissue)
- Year:
- 2024
- Journal Name:
- Scientific Reports
- PubMed ID:
- 38467789
Human umbilical cord-derived mesenchymal stem cells improve the function of liver in rats with acute-on-chronic liver failure via downregulating Notch and Stat1/Stat3 signaling
- Species:
- Rat
- Sample Type:
- Serum (acute-on-chronic liver failure rats)
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 34256837
The hepatoprotective effect of gooseberry and black mulberry extracts against carbon tetrachloride-induced liver injury in rats
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2021
- Journal Name:
- The Journal of Basic and Applied Zoology
Nanoparticle-Mediated Delivery of Emodin via Colonic Irrigation Attenuates Renal Injury in 5/6 Nephrectomized Rats
- Species:
- Rat
- Sample Type:
- Serum; Tissue Lysate / Homogenate (renal tissue)
- Year:
- 2020
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 33551808
Icariin Protects Hippocampal Neurons From Endoplasmic Reticulum Stress and NF-κB Mediated Apoptosis in Fetal Rat Hippocampal Neurons and Asthma Rats
- Species:
- Rat
- Sample Type:
- Serum (asthmatic rats, icariin-treated)
- Year:
- 2019
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 32082160
Huoxiang Zhengqi Oral Liquid Attenuates LPS-Induced Acute Lung Injury by Modulating Short-Chain Fatty Acid Levels and TLR4/NF-κB p65 Pathway
- Species:
- Rat
- Sample Type:
- Serum, bronchoalveolar lavage fluid (BALF)
- Year:
- 2023
- Journal Name:
- BioMed research international
Supercritical Fluid Extract of Angelica sinensis and Zingiber officinale Roscoe Ameliorates TNBS-Induced Colitis in Rats
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (splenocyte culture supernatants)
- Year:
- 2019
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 31387229
Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats
- Species:
- Rat
- Sample Type:
- Serum (Liver injury model, wistar rats)
- Year:
- 2015
- Journal Name:
- Life Sciences
- PubMed ID:
- 26165752
Evaluation of antitumour activity of tea carbohydrate polymers in hepatocellular carcinoma animals
- Species:
- Human
- Sample Type:
- Serum (Hepatocellular carcinoma rat model treated with tea carbohydrate)
- Year:
- 2012
- Journal Name:
- International Journal of Biological Macromolecules
- PubMed ID:
- 22406869
Longer period of oral administration of aspartame on cytokine response in Wistar albino rats
- Species:
- Rat
- Sample Type:
- Serum (aspartame treated wistar rats)
- Year:
- 2015
- Journal Name:
- Endocrinología y Nutrición
- PubMed ID:
- 25681123
