Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Rat Adiponectin ELISA Kit
Rat Adiponectin ELISA Kit
RayBio® Rat Adiponectin (ACRP30) ELISA Kit for Cell Culture Supernatants and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 0.8 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.8 ng/ml - 200 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, CD80, CD48, CTACK, Decorin, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1, TWEAK R. |
| Compatible Sample Types | Cell Culture Supernatants, Serum |
| Recommended Dilution (Serum/Plasma) | 300 fold |
| Accession Number | Q8K3R4 |
| Gene Id | 246253 |
| Gene Symbols | ADIPOQ, ACDC, RCG_36676 |
| Protein Name / Synonyms | 30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing) (Protein Adipoq) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Rat Adiponectin qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat Adiponectin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Adiponectin (ACRP30 / ADIPOQ), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Thymoquinone controlled obesity and invigorated cognitive and memory performance in rats consuming a high-fat diet via modulating oxidative stress, inflammation and apoptosis
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40542063
Catechins prevent obesity-induced kidney damage by modulating PPARγ/CD36 pathway and gut-kidney axis in rats
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
The Role of Lymph-Adipose Crosstalk in Alcohol-Induced Perilymphatic Adipose Tissue Dysfunction
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (PLAT, SFAT tissue homogenates)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39409139
Enhanced mitigation of diabetic myocardial injury in rats via modulating the adiponectin/IRS-1/PI3K/p-AKT/FOXO-1 signaling by combining methotrexate and insulin
- Year:
- 2026
- Journal Name:
- Molecular Biology Reports
- PubMed ID:
- 42631883
Anti-Obesity Effect of a Tea Mixture Nano-Formulation on Rats Occurs via the Upregulation of AMP-Activated Protein Kinase/Sirtuin-1/Glucose Transporter Type 4 and Peroxisome Proliferator-Activated Receptor Gamma Pathways
- Species:
- Rat
- Sample Type:
- adipose tissue
- Year:
- 2023
- Journal Name:
- Metabolites
Assessment of anti-psoriatic activity of bakuchiol-loaded solid lipid nanoparticles-based gel: design, characterization, and mechanistic insight via NF-kB signaling pathway
- Species:
- Rat
- Sample Type:
- skin tissue
- Year:
- 2023
- Journal Name:
- Naunyn-schmiedebergs Archives of Pharmacology
Modulatory Effect of 4-(methylthio)butyl Isothiocyanate Isolated From Eruca Sativa Thell. on DMBA Induced Overexpression of Hypoxia and Glycolytic Pathway in Sprague-Dawley Female Rats
- Species:
- Rat
- Sample Type:
- Plasma (fresh plasma)
- Year:
- 2021
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 34447314
β-Sitosterol Circumvents Obesity Induced Inflammation and Insulin Resistance by down-Regulating IKKβ/NF-κB and JNK Signaling Pathway in Adipocytes of Type 2 Diabetic Rats
- Species:
- Rat
- Sample Type:
- Serum (high-fat diet/STZ type 2 diabetic rats)
- Year:
- 2021
- Journal Name:
- Molecules
- PubMed ID:
- 33917607
Antidiabetic and antihyperlipidemic effects of aqueous extract of Parquetina nigrescens in streptozotocin-nicotinamide induced type 2 diabetic rats
- Species:
- Rat
- Sample Type:
- Serum (STZ-nicotinamide type 2 diabetic rats)
- Year:
- 2021
- Journal Name:
- Heliyon
- PubMed ID:
- 34222697
The Long-Term Effects of High-Fat and High-Protein Diets on the Metabolic and Endocrine Activity of Adipocytes in Rats
- Species:
- Rat
- Sample Type:
- Serum (incubation medium)
- Year:
- 2021
- Journal Name:
- Biology-Basel
- PubMed ID:
- 33920712
