RALY (NM_016732) Human Tagged ORF Clone
Referência RC210723
Tamanho : 10ug
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RALY (NM_016732) Human Tagged ORF Clone
SKU
RC210723
RALY (Myc-DDK-tagged)-Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RALY |
| Synonyms | HNRPCL2; P542 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_016732 |
| ORF Size | 921 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016732.3 |
| RefSeq Size | 4790 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 22913 |
| UniProt ID | Q9UKM9, Q53GL6 |
| Cytogenetics | 20q11.22 |
| Domains | RRM |
| MW | 32.6 kDa |
| Summary | This gene encodes a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) gene family. This protein may play a role in pre-mRNA splicing and in embryonic development. Alternate splicing results in multiple transcript variants. provided by RefSeq, Sep 2011 |
| Product Manuals |
| FAQs |
|
| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC210723L1 | Lenti ORF clone of Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC210723L2 | Lenti ORF clone of Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1, mGFP tagged | 10 ug | | |
| RC210723L3 | Lenti ORF clone of Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC210723L4 | Lenti ORF clone of Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1, mGFP tagged | 10 ug | | |
| RG210723 | RALY (tGFP-tagged) - Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1 | 10 ug | | |
| SC114148 | RALY (untagged)-Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1 | 10 ug | | |
| SC322780 | RALY (untagged)-Human RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) (RALY), transcript variant 1 | 10 ug | | |
Citations
CCR5AS lncRNA variation differentially regulates CCR5, influencing HIV disease outcome
Kulkarni, Lied, Kulkarni et al
Nat. Immunol. (2019) 20 (7), 824-834 DOI: 10.1038/s41590-019-0406-1
Kulkarni, Lied, Kulkarni et al
Nat. Immunol. (2019) 20 (7), 824-834 DOI: 10.1038/s41590-019-0406-1
AI Summary: In this study, the OriGene Raly cDNA Myc-tagged plasmid (clone RC210723) was used to investigate the role of the RNA-binding protein Raly in regulating CCR5 mRNA stability and its impact on HIV infection.
Snippet: Hut-78 were transfected with a plasmid encoding Raly cDNA with a Myc-tag (clone RC210723;Origene) along with either siCon1 or siLnc1. For RIP of ribonucleoprotein complexes from whole-cell extracts, the transfected cells were lysed in 20 mM Tris–HCl at pH 7.5, 100 mM KCl, 5 mM MgCl2 and 0.5% Nonidet P-40 for 10 min on ice and centrifuged at 10,000 r.p.m. for 15 min at 4 °C
The hnRNP RALY regulates transcription and cell proliferation by modulating the expression of specific factors, including the proliferation-marker E2F1
Cornella, Tebaldi, Gasperini et al
J. Biol. Chem. (2017)
Cornella, Tebaldi, Gasperini et al
J. Biol. Chem. (2017)
AI Summary: OriGene’s pCMV6-Entry Vector was used to clone human RALY cDNA with a c-Myc/DDK tag, enabling the study of RALY’s role in regulating gene expression and cell proliferation in this research.
Snippet: Constructs. Reverse transcription was performed on total RNA isolated from HeLa cells using the TRIzol Reagent. Human RALY cDNA was amplified with the Fusion High-Fidelity DNA polymerase (New England BioLabs), and then cloned in frame with the c-Myc/DDK tag (pCMV6-Entry Vector, Origene). The sequences of the primers are listed in the Supplementary Table S3
Heterogeneous nuclear ribonucleoprotein C proteins interact with the human papillomavirus type 16 (HPV16) early 3'-untranslated region and alleviate suppression of HPV16 late L1 mRNA splicing
Dhanjal, Kajitani, Glahder et al
J. Biol. Chem. (2015)
Dhanjal, Kajitani, Glahder et al
J. Biol. Chem. (2015)
AI Summary: OriGene products contributed to this study by providing CMV promoter-driven expression plasmids for myc-tagged-RALY (RC210723) and myc-tagged-RALYL (RC206313), which were used to investigate their roles in regulating HPV16 late gene expression.
Snippet: CMV promoter-driven expression plasmids for myc-tagged-RALY (RC210723) and myc-tagged-RALYL (RC206313) were purchased from Origene. To construct RALYL deletion mutant pCRALYL-C the N-terminus was PCR ... Cite SaveSaving
Showing 1-3 of 3 papers.
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