Rabbit IgG (H+L), Mouse/Human ads Antibody : TRITC

Referência OASB01973

Tamanho : 1.0mg

Marca : Aviva Systems Biology

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Goat Anti-Rabbit IgG(H+L) Antibody - TRITC Conjugated (OASB01973)

Datasheets/ManualsPrintable datasheet for Goat Anti-Rabbit IgG(H+L) Antibody - TRITC Conjugated (OASB01973)
Product Info
Tested Species ReactivityRabbit
Predicted Species ReactivityRabbit
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationTRITC
ApplicationFLISA, IHC-F, IHC-P, IHC, ICC
Additional InformationDescription: Pooled antisera from goats hyperimmunized with rabbit IgG; purified by affinity chromatography on pooled rabbit IgG covalently linked to agarose; reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM; cross-adsorbed against mouse and human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenRabbit IgG
PurificationAffinity chromatography on pooled IgG covalently linked to agarose.
Concentration1.0 mg/mL
SpecificityIgG(H+L)
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoicacid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
FLISA:
ELISA:
FITC and BIOT conjugates
R-PE, APC and SPRD conjugates
TRITC and TXRD conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:100-1:400
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoDirect and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mg in1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The Beta- galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS/NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.5 mg in 1.0 mL or0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionPooled human sera, purified human paraproteins, pooled mouse sera, andpooled mouse plasmacytoma/hybridoma proteins.
Gene Full NameIg gamma-1 chain C region