Rabbit IgG Antibody : PE

Referência OASB01965

Tamanho : 0.5mg

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Rabbit IgG Antibody : PE (OASB01965)
Product Info
Tested Species ReactivityRabbit
Predicted Species ReactivityRabbit
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationR-PE
ApplicationFLISA, FC
Additional InformationDescription: Pooled antisera from goats hyperimmunized with rabbit IgG; purified by affinity chromatography on pooled rabbit IgG covalently linked to agarose; reacts with the heavy chain of rabbit IgG; cross-adsorbed against rabbit IgM for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenRabbit IgG
PurificationAffinity chromatography on rabbit IgG covalently linked to agarose
Concentration0.5 mg/mL
SpecificityIgG
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA or FLISA for conformance tocharacteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFLISA:
ELISA:
FITC conjugate
TRITC conjugate
R-PE conjugate
AP conjugate
HRP conjugate
BIOT conjugate
1:200 – 1:400
1:100 – 1:400
1 ug/mL
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoDirect and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3
Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionRabbit IgM
Gene SymbolLOC100009097
Gene Full NameIg gamma-1 chain C region
Alias SymbolsIGGC, ighg
NCBI Gene Id100009097
Protein NameIg gamma chain C region
Uniprot IDP01870