Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit
Referência CSB-EL002736RB-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
bone morphogenetic protein 2
Associated names
BMP2 ELISA Kit; BMP-2Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit
Abbreviation
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
0.39 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 80-94 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:10 | Average % | 95 | |||||
| Range % | 84-102 | ||||||
Recovery
| The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 82-100 | |||||
| EDTA plasma (n=4) | 97 | 88-105 | |||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rabbit BMP2 ELISA Kit was designed for the quantitative measurement of Rabbit BMP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.
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Target Background
Function
(From Uniprot)
(From Uniprot)
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes....
Gene References into Functions
- The transfecting capability of a BMP-2 specific vector is examined and supports the idea that BMP-2 might diminish osseointegration and implant fixation.
- While BMP2 expression begins at (pregastrulation) stage 1 in the hypoblast, BMP4 expression commences--distinctly delayed compared to the mouse--diffusely at (pregastrulation) stage 2; from stage 3 onwards.
- Maxillary sinus floor elevation using BMP-2 gene-modified bone marrow stromal cells and TCP in rabbits
- Data show that BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes.
- BMP2 is not suitable to regenerate osteochondral lesions completely
Subcellular Location
Secreted.
Protein Families
TGF-beta family
Database Links
KEGG:
STRING:
UniGene: