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> Rabbit Anti-ZEB1 Polyclonal Antibody

Rabbit Anti-ZEB1 Polyclonal Antibody

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Referência HPA027524

Tamanho : 100ul

Marca : Atlas Antibodies

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Telefone : +1 850 650 7790

HPA027524
zinc finger E-box binding homeobox 1

Anti-ZEB1 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN ZEB1

0.2 mg/ml
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA027524 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: zinc finger E-box binding homeobox 1
  • Target gene: ZEB1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000024238 (74%)RAT - ENSRNOG00000017863 (76%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000041609
  • Concentration: 0.2 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  

Target information

  • Protein name: zinc finger E-box binding homeobox 1
  • Gene name: ZEB1
  • Alternative gene names: AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (119)

  • Peters NA, Heinz MC, Küçükköse E, Viergever BJ, Baars M, Amini M, Huismans MA, Andel D, Verheem A, Vercoulen Y, Snippert H, Borel Rinkes IH, Kranenburg OJ Transl Med , 2025 Dec 3; 24:37. Epub 2025 Dec 32025 Dec 3
  • Vandenbussche N, Imschoot R, Lintermans B, Denolf L, Taminau J, Fieuws C, Berx G, Gevaert K, Claes KBMol Oncol , 2025 Jun 18; 19(11):3360-3375. Epub 2025 Jun 182025 Jun 18