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> Rabbit Anti-TUFM Polyclonal Antibody

Rabbit Anti-TUFM Polyclonal Antibody

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Referência HPA018991

Tamanho : 100ul

Marca : Atlas Antibodies

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HPA018991
Tu translation elongation factor, mitochondrial

Anti-TUFM Antibody

Polyclonal Antibody against HUMAN TUFM

0.2 mg/ml
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
Anti-TUFM Antibody
info@quimigen.pt

Immunohistochemical staining of human duodenum, endometrium, kidney and testis using Anti-TUFM antibody HPA018991 (A) shows similar protein distribution across tissues to independent antibody HPA024087 (B).

Product details

  • Target protein: Tu translation elongation factor, mitochondrial
  • Target gene: TUFM
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000073838 (89%)RAT - ENSRNOG00000018604 (89%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A118130
  • Concentration: 0.2 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody   
  • Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. 

Target information

  • Protein name: Tu translation elongation factor, mitochondrial
  • Gene name: TUFM
  • Alternative gene names: EF-TuMT, EFTu, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Christensen IB, Wu Q, Bohlbro AS, Skals MG, Damkier HH, Hübner CA, Fenton RA, Praetorius JFluids Barriers CNS , 2020 Jan 7; 17:2. Epub 2020 Jan 72020 Jan 7
  • Lei Y, Wen H, Yu Y, Taxman DJ, Zhang L, Widman DG, Swanson KV, Wen KW, Damania B, Moore CB, Giguère PM, Siderovski DP, Hiscott J, Razani B, Semenkovich CF, Chen X, Ting JPImmunity , 2012 Jun 29; 36(6):933-462012 Jun 29

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