Rabbit Anti-TOMM70 Polyclonal Antibody

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Referência HPA014589

Tamanho : 100ul

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Telefone : +1 850 650 7790

Marca : Atlas Antibodies

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

HPA014589

translocase of outer mitochondrial membrane 70

Anti-TOMM70 Antibody

Polyclonal Antibody against HUMAN TOMM70

0.1 mg/ml

info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using HPA014589 antibody. Corresponding TOMM70 RNA-seq data are presented for the same tissues.

Product details

Target protein: translocase of outer mitochondrial membrane 70
Target gene: TOMM70
Verified species reactivity: Human, Mouse
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000022752 (92%)RAT - ENSRNOG00000001640 (92%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000020129
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: translocase of outer mitochondrial membrane 70
Gene name: TOMM70
Alternative gene names: KIAA0719, Tom70, TOMM70A,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

Islam R, Roche ME, Lin Z, Whitaker‐Menezes D, Diaz‐Barros V, Serrano E, Martinez Cantarin MP, Philp NJ, Basu Mallick A, Martinez‐Outschoorn UMol Oncol , 2025 Feb 25; 19(8):2431-2455. Epub 2025 Feb 252025 Feb 25
Moschandrea C, Kondylis V, Evangelakos I, Herholz M, Schneider F, Schmidt C, Yang M, Ehret S, Heine M, Jaeckstein MY, Szczepanowska K, Schwarzer R, Baumann L, Bock T, Nikitopoulou E, Brodesser S, Krüger M, Frezza C, Heeren J, Trifunovic A, Pasparakis MNature , 2023 Dec 20; 625(7994):385-392. Epub 2023 Dec 202023 Dec 20