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> Rabbit Anti-OLIG2 Polyclonal Antibody

Rabbit Anti-OLIG2 Polyclonal Antibody

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Referência HPA003254

Tamanho : 100ul

Marca : Atlas Antibodies

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Telefone : +1 850 650 7790

HPA003254
oligodendrocyte lineage transcription factor 2

Anti-OLIG2 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN OLIG2

0.3 mg/ml
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
Anti-OLIG2 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA003254 antibody. Corresponding OLIG2 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: oligodendrocyte lineage transcription factor 2
  • Target gene: OLIG2
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000039830 (93%)RAT - ENSRNOG00000028658 (94%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000047004
  • Concentration: 0.3 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:2500 - 1:5000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: oligodendrocyte lineage transcription factor 2
  • Gene name: OLIG2
  • Alternative gene names: BHLHB1, bHLHe19, OLIGO2, PRKCBP2, RACK17, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (13)

  • Kazakou NL, Bestard-Cuche N, Wagstaff LJ, Horan K, Seeker L, Bøstrand S, Fetit R, Smith RS, Pohl FB, Neumann B, Keeler P, Franklin RJ, Williams ANat Commun , 2025 Aug 30; 16:8126. Epub 2025 Aug 302025 Aug 30
  • Rajani RM, Ellingford R, Hellmuth M, Harris SS, Taso OS, Graykowski D, Lam FK, Arber C, Fertan E, Danial JS, Swire M, Lloyd M, Giovannucci TA, Bourdenx M, Klenerman D, Vassar R, Wray S, Sala Frigerio C, Busche MAPLoS Biol , 2024 Jul 23; 22(7):e3002727. Epub 2024 Jul 232024 Jul 23