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> Rabbit Anti-JUN Polyclonal Antibody

Rabbit Anti-JUN Polyclonal Antibody

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Referência HPA059474

Tamanho : 100ul

Marca : Atlas Antibodies

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HPA059474
jun proto-oncogene

Anti-JUN Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN JUN

0.1 mg/ml
Anti-JUN Antibody
Anti-JUN Antibody
Anti-JUN Antibody
Anti-JUN Antibody
Anti-JUN Antibody
Anti-JUN Antibody
Anti-JUN Antibody
info@quimigen.pt

Western blot analysis in human cell lines HEK293 and HeLa using Anti-JUN antibody. Corresponding JUN RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Product details

  • Target protein: jun proto-oncogene
  • Target gene: JUN
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000052684 (97%)RAT - ENSRNOG00000026293 (95%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: R83086
  • Concentration: 0.1 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. 
  • Immunofluorescence in Cell Lines (ICC-IF) 

Target information

  • Protein name: jun proto-oncogene
  • Gene name: JUN
  • Alternative gene names: AP-1, c-Jun, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Zhao L, Cheng Z, Lu Z, Jin JTransl Cancer Res , 2021 Mar; 10(3):1457-14692021 Mar
  • Tao A, Wang X, Li CCancer Manag Res , 2021 Jan 26; 13:723-732. Epub 2021 Jan 262021 Jan 26