rabbit Anti-IgM (mu chain specific) (AP) Polyclonal Antibody

Referência I1905-03Q-1mg

Tamanho : 1mg

Marca : US Biological

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I1905-03Q IgM (mu chain specific) (AP)

Clone Type
Polyclonal
Host
rabbit
Source
rat
Isotype
IgM
Grade
Affinity Purified
Applications
DB E IHC WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000 to 1:3,000, assayed against 1.0ug of Rat IgM in a standard capture using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.

Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgM|Host: rabbit|Source: rat|Concentration: ~1.0 mg/ml|Form: Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50%|Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Rat IgM heavy chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine, anti-Rabbit Serum, Rat IgM, and Rat Serum. No reaction was observed against Rat IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgM heavy chain
Form
Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine, anti-Rabbit Serum, Rat IgM, and Rat Serum. No reaction was observed against Rat IgG F(c).
References
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.