rabbit Anti-FABP5 (Fatty Acid Binding Protein 5, Epidermal, E-FABP, EFABP, PA-FABP, PAFABP, Psoriasis-Associated, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog) Polyclonal Antibody

Fatty acid binding protein 5 was microsequenced, and degenerate oligonucleotides based on the amino acid sequence were used to screen a lambda-gt11 cDNA library prepared from noncultured psoriatic keratinocytes. The PAFABP cDNA encodes a 135aa protein with molecular weight 15,164. By Northern blot analysis, the authors detected elevated levels of PAFABP message in noncultured psoriatic keratinocytes as compared to normal keratinocytes. PAFABP mRNA was also observed in transformed human epithelial cell lines, A431 epidermoid carcinoma cells, transformed human amnion (AMA) cells, and lymphoid Molt 4 cells. PAFABP protein was increased in primary normal keratinocytes induced to differentiate, as determined by 2D gel electrophoresis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.