R-CHP | Collagen Hybridizing Peptide,Cy3 Conjugate

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Tamanho : 300µg+60µgNegativeControlKit

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Description

Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.^[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.^[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.^[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.^[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.^[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.^[7]

R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).^[3,7]

Applications: immunofluorescence,^[3] cell imaging,^[4] SDS-PAGE (in-gel western)^[7]

Features

rmative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
A non-antibody approach with no species restrictions against any co-staining antibody
Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
Stable in solution under 4 °C, eliminating the need to aliquot for storage

For research use only. Not intended or approved for diagnostic or therapeutic use.

Fei, Y., et al. (2024). Promoting Chondrogenesis by Targeted Delivery to the Degenerating Cartilage in Early Treatment of Osteoarthritis. Bioactive Materials, 40, 624–633. doi: 10.1016/j.bioactmat.2024.08.004
Fang, Y., et al. (2024). Collagen Denaturation in Post-Run Achilles Tendons and Achilles Tendinopathy: In Vivo Mechanophysiology and Magnetic Resonance Imaging. Science Advances, 10(40), eado2015. doi: 10.1126/sciadv.ado2015
Rappold, R., et al. (2024). Salmonella‐Driven Intestinal Edema in Mice is Characterized by Tensed Fibronectin Fibers. The FEBS Journal. Advance online publication. doi: 10.1111/febs.17190
Dhiman, M. S., et al. (2024). Collagen Integrity of the Annulus Fibrosus in Degenerative Disc Disease Individuals Quantified with Collagen Hybridizing Peptide. JOR Spine, 7(3), e1359. doi: 10.1002/jsp2.1359
Satoh, A., et al. (2024). Keratinocyte-Driven Dermal Collagen Formation in the Axolotl Skin. Developmental Biology, 509, 56–66. doi: 10.1016/j.ydbio.2024.03.003
Zhao, J., et al. (2024). Delineating, Imaging, and Assessing Pulmonary Fibrosis Remodeling via Collagen Hybridization. ACS Nano, 18(41), 27997–28011. doi: 10.1021/acsnano.4c06139
Ahmad, A., et al. (2024). Molecular Imaging of Collagen Remodeling in a Murine Model of Lung Fibrosis. bioRxiv. Preprint. doi: 10.1101/2024.03.01.582999
Wang, H., et al. (2024). Chiral Arginine Modified Electrospun Membrane for Enhancing Tendon Healing. Advanced Functional Materials, 2402845. doi: 10.1002/adfm.202402845
Xiao, Z., et al. (2023). Desmoplastic Stroma Restricts T Cell Extravasation and Mediates Immune Exclusion and Immunosuppression in Solid Tumors. bioRxiv. Preprint. doi: 10.1101/2023.04.13.536777
Loflin, B. E., et al. (2023). An Adolescent Murine In Vivo Anterior Cruciate Ligament Overuse Injury Model. The American Journal of Sports Medicine, 51(7), 1721–1732. doi: 10.1177/03635465231165753
Smith, K. A., et al. (2023). Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development. Journal of Cardiovascular Translational Research, 16(2), 463–472. doi: 10.1007/s12265-022-10316-y
Salarian, M., et al. (2023). Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity. Circulation Research, 132(4), 432–448. doi: 10.1161/CIRCRESAHA.122.322096
Flinn, M. A., et al. (2023). Myofibroblast Ccn3 is Regulated by Yap and Wwtr1 and Contributes to Adverse Cardiac Outcomes. Frontiers in Cardiovascular Medicine, 10, 1142612. doi: 10.3389/fcvm.2023.1142612
Lim, P. J., et al. (2023). Perturbations in Fatty Acid Metabolism and Collagen Production Infer Pathogenicity of a Novel MBTPS2 Variant in Osteogenesis Imperfecta. Frontiers in Endocrinology, 14, 1195704. doi: 10.3389/fendo.2023.1195704
Ho, Y.-C., et al. (2023). PROX1 Inhibits PDGF-B Expression to Prevent Myxomatous Degeneration of Heart Valves. Circulation Research, 133(6), 463–480. doi: 10.1161/CIRCRESAHA.123.323027
Nishi, K., et al. (2023). A Thioacetamide-Induced Liver Fibrosis Model for Pre-Clinical Studies in Microminipig. Scientific Reports, 13(1), 14996. doi: 10.1038/s41598-023-42144-8
Zhang, Y., et al. (2023). Omentin-1 Induces Mechanically Activated Fibroblasts Lipogenic Differentiation through PKM2/YAP/PPARγ Pathway to Promote Lung Fibrosis Resolution. Cellular and Molecular Life Sciences, 80(10), 308. doi: 10.1007/s00018-023-04961-y
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Sun, A. R., et al. (2023). Hybrid Hydrogel-Extracellular Matrix Scaffolds Identify Distinct Ligand and Mechanical Signatures in Cardiac Aging. bioRxiv. Preprint. doi: 10.1101/2023.10.11.561941
Jones, B., et al. (2022). Structurally Abnormal Collagen Fibrils in Abdominal Aortic Aneurysm Resist Platelet Adhesion. Journal of Thrombosis and Haemostasis, 20(2), 470–477. doi: 10.1111/jth.15576
Smith, K. A. (2022). Evaluation of Hemodynamic Induced Alterations in Extracellular Matrix Organization in Atherogenesis [Doctoral dissertation, The University of Utah]. University of Utah Research Repository. Link to dissertation
Atta, G. (2022). The Lymphogenic and Hemangiogenic Factors Affecting the Human Sclera [Doctoral dissertation, Universität zu Köln]. Kölner UniversitätsPublikationsServer. Link to dissertation
Kim, M., et al. (2022). A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate. Biomolecules & Therapeutics, 30(2), 126–133. doi: 10.4062/biomolther.2021.139

Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP), Product Vial

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

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Synonyms	R-CHP, collagen mimetic peptide (CMP)
Molecular weight	3191.44 g/mol
Purity	85% by HPLC
Conjugate	Single sulfo-Cyanine3 tag per peptide
Excitation	548 nm
Emission	563 nm
Content	Purified lyophilized powder
Storage	-20 °C as powder, 4 °C after reconstitution in water

R-CHP | Collagen Hybridizing Peptide,Cy3 Conjugate

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R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate