E-OSEL-Ch0003-48T | QuicKey Pro Chicken T (Testosterone) ELISA Kit

Size	Price	Qty
96T	$ 520.00	- +
48T	$ 416.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.13 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Chicken
Specificity	This kit recognizes Chicken T in samples.No significant cross-reactivity or interference between Chicken T and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Performance

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken T are added to the micro ELISA plate wells. Chicken T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Chicken T in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.

Research Area

Signal Transduction , Signal Transduction , Epigenetics And Nuclear Signaling , Developmental Biology

QuicKey Pro Chicken T(Testosterone) ELISA Kit - procedures

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Videos

QuicKey Pro ELISA (Sandwich Method) Operation Guide Video

QuicKey Pro ELISA (Competitive Method) Operation Guide Video

Resources

• Save Time, Boost Accuracy: Why Researchers Are Switching to QuicKey Pro™ (One-Step) ELISA Kits • ELISA Sample Processing Methods • ELISA Explained: How It Works and How to Interpret Results with Standard Curve Analysis