Pyruvate Fluorometric Assay Kit
Referência E-BC-F058-48T
Tamanho : 48T
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
For research use only.
| Detection Principle | Pyruvate is an important component of the organism. It mainly participates in the metabolism of sugar and fat, and is also one of the intermediate products of carbohydrate metabolism. The detection principle of this kit is that pyruvate undergoes a series of reactions under the action of enzyme reagents, and the resulting substance can react with the color developer. The content of pyruvate in the sample is calculated by measuring the fluorescence value of excitation wavelength 535 nm and emission wavelength 587 nm. |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.00 μmol/L |
| Detection Range | 1.00-100 μmol/L |
| Precision | inter-assay CV: 2.0-4.0% | intra-assay CV: 1.0-2.0% |
| Sample Volume | 100-150 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Guinea pig serum | 1 |
| Rabbit serum | 1-2 |
| Human serum | 1 |
| Rat serum | 1-2 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 1×10^6 239T cells | 1 |
| 1×10^6 CHO cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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