Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.

A549 cells:300 nM, K562 cells:0.22 μM, KB-V1 cells:79.3 μM, KB-3-1 cells:0.21 μM, HepG2 cells:1600 μM, PRH cells:2000 nM, MOLT-4 cells:0.17 μM (EC50), HEK293T cells:0.42 μM (CC50), J774.A1 cells:10 μM, HEK293 cells:0.35 nM

METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1]
METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection.
RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2]
METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL).
RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3]

Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.

H2O: 92 mg/mL (168.98 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 255 mg/mL (468.37 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (9.18 mM), Sonication is recommended.