PTPN22 (NM_015967) Human Tagged ORF Clone
Referência RC218969
Tamanho : 10ug
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PTPN22 (NM_015967) Human Tagged ORF Clone
SKU
RC218969
PTPN22 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PTPN22 |
| Synonyms | LYP; LYP1; LYP2; PEP; PTPN8; PTPN22.5; PTPN22.6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_015967 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015967.7 |
| RefSeq Size | 3615 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 26191 |
| UniProt ID | Q9Y2R2 |
| Cytogenetics | 1p13.2 |
| Domains | PTPc_motif, Y_phosphatase |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 91.7 kDa |
| Summary | This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. provided by RefSeq, Mar 2009 |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC218969L1 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC218969L2 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, mGFP tagged | 10 ug | | |
| RC218969L3 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC218969L4 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, mGFP tagged | 10 ug | | |
| RG218969 | PTPN22 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1 | 10 ug | | |
| SC114550 | PTPN22 (untagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1 | 10 ug | | |
Plasmid Map for RC218969
Download sequence for
Cloning scheme for RC218969
Circular map for RC218969
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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