PTHLH ELISA Kit (Bovine)
Referência OKEH07127
Tamanho : 96Wells
Marca : Aviva Systems Biology
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PTHLH ELISA Kit (Bovine) (OKEH07127)
| Datasheets/Manuals | Printable datasheet for PTHLH ELISA Kit (Bovine) (OKEH07127) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Pthlh ELISA Kit (Bovine) (OKEH07127) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pthlh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pthlh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pthlh captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 39 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Parathyroid hormone-related protein | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | PTHLH |
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| Gene Full Name | parathyroid hormone like hormone |
| Alias Symbols | parathyroid hormone-related protein, PTH-rP, PTHrP. |
| NCBI Gene Id | 286767 |
| Protein Name | Parathyroid hormone-related protein |
| Description of Target | Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). |
| Uniprot ID | P58073 |
| Protein Accession # | NP_777178 |
| Nucleotide Accession # | NM_174753 |
