Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (AP)
Referência MBS6241941-0,1mL
Tamanho : 0.1mL
Marca : MyBiosource
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Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (AP)
Western Blot Analysis: Representative lot data. Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1), Lane 1, and a strain lacking the methylestrase, delta PPE1, Lane 2, were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-methyl PP2A, C subunit (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates methylated-PP2A (~36kD).
Protein Phosphatase 2 (PP2Ac) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6241941MBS6241941 is an antibody targeting PP2Ac. Also known as Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (AP).… More details
Product Overview
Online Datasheet in PDFProduct Name
Protein Phosphatase 2 (PP2Ac), Monoclonal Antibody
Full Product Name
Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (AP)
Product Gene Name
anti-PP2Ac antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
9i192
Host
Mouse
Species Reactivity
Human, Mouse, Yeast
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes the methylated human form of PP2A, catalytic subunit. Species crossreactivity: mouse, yeast. Species sequence homology: rat, pig, rabbit, chicken, Drosophila.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Molecular Weight
35,594 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (133532148..133561950, complement). Location: 5q31.1
OMIM
176915
Immunogen
Synthetic peptide (C-betaA-RRTPDYFL-Ome) corresponding to aa302-309 of human PP2A catalytic subunit (KLH). Epitope: Methyl-esterified on Leu309
Conjugate
AP
Product Categories/Family for anti-PP2Ac antibody
Antibodies; Enzymes; Phosphatase
Applications Tested/Suitable for anti-PP2Ac antibody
Western Blot (WB)
Western Blot (WB) of anti-PP2Ac antibody
Western Blot Analysis: Representative lot data. Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1), Lane 1, and a strain lacking the methylestrase, delta PPE1, Lane 2, were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-methyl PP2A, C subunit (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates methylated-PP2A (~36kD).
Product References and Citations for anti-PP2Ac antibody
1. Palanivel, R., et al. (2004). Am. J. Physiol. Endocrinol. Metab. 286: E1032-E1041. 2. Guo, C., et al. (2000). J. Biol. Chem. 277: 4839-4844. 3. Ogris, E., et al. (1999). J. Biol. Chem. 274: 14382-14391. 4. Turowski, P., et al. (1995). J. Cell Biol. 129: 397-410.
Other Notes
Small volumes of anti-PP2Ac antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PP2Ac. It may not necessarily be applicable to this product.
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
NCBI Official Synonym Full Names
protein phosphatase 2, catalytic subunit, alpha isozyme
NCBI Official Symbol
PPP2CA
NCBI Official Synonym Symbols
RP-C; PP2Ac; PP2CA; PP2Calpha
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Synonym Protein Names
Replication protein C
Protein Family
Protein phosphatase 2B regulatory
UniProt Gene Name
PPP2CA
UniProt Synonym Gene Names
PP2A-alpha; RP-C
UniProt Entry Name
PP2AA_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine pr
NCBI Summary for PP2Ac
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for PP2Ac
PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region
Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity
Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process
Show more Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region
Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity
Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-PP2Ac antibody
| Reference Product | PubMed Publications |
|---|---|
| SET antibody | >72 publications with PP2Ac and SET |
| PPP2R1B antibody | >60 publications with PP2Ac and PPP2R1B |
| MAPK1 antibody | >59 publications with PP2Ac and MAPK1 |
| MAPK3 antibody | >47 publications with PP2Ac and MAPK3 |
| AKT1 antibody | >27 publications with PP2Ac and AKT1 |
| PPP2R2A antibody | >24 publications with PP2Ac and PPP2R2A |
| PPP2R1A antibody | >23 publications with PP2Ac and PPP2R1A |
| APC antibody | >21 publications with PP2Ac and APC |
| RHO antibody | >21 publications with PP2Ac and RHO |
| JAK2 antibody | >18 publications with PP2Ac and JAK2 |
Pathways associated with anti-PP2Ac antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Activated TLR4 Signalling Pathway antibodies | Activated TLR4 Signalling Pathway Diagram |
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| BDNF Signaling Pathway antibodies | BDNF Signaling Pathway Diagram |
| Beta-catenin Phosphorylation Cascade Pathway antibodies | Beta-catenin Phosphorylation Cascade Pathway Diagram |
| C-MYC Pathway antibodies | C-MYC Pathway Diagram |
| CTLA4 Inhibitory Signaling Pathway antibodies | CTLA4 Inhibitory Signaling Pathway Diagram |
| Canonical Wnt Signaling Pathway antibodies | Canonical Wnt Signaling Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| Chagas Disease (American Trypanosomiasis) Pathway antibodies | Chagas Disease (American Trypanosomiasis) Pathway Diagram |
Diseases associated with anti-PP2Ac antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >411 publications with PP2Ac and Neoplasms |
| Nervous System Diseases Antibodies | >162 publications with PP2Ac and Nervous System Diseases |
| Cardiovascular Diseases Antibodies | >98 publications with PP2Ac and Cardiovascular Diseases |
| Drug Toxicity Antibodies | >91 publications with PP2Ac and Drug Toxicity |
| Necrosis Antibodies | >64 publications with PP2Ac and Necrosis |
| Heart Diseases Antibodies | >58 publications with PP2Ac and Heart Diseases |
| Neoplasms, Experimental Antibodies | >46 publications with PP2Ac and Neoplasms, Experimental |
| Lung Diseases Antibodies | >46 publications with PP2Ac and Lung Diseases |
| Inflammation Antibodies | >45 publications with PP2Ac and Inflammation |
| Liver Diseases Antibodies | >32 publications with PP2Ac and Liver Diseases |
Organs/Tissues associated with anti-PP2Ac antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >362 publications with PP2Ac and Brain |
| Muscle Antibodies | >264 publications with PP2Ac and Muscle |
| Blood Antibodies | >237 publications with PP2Ac and Blood |
| Liver Antibodies | >154 publications with PP2Ac and Liver |
| Heart Antibodies | >153 publications with PP2Ac and Heart |
| Nerve Antibodies | >121 publications with PP2Ac and Nerve |
| Lung Antibodies | >95 publications with PP2Ac and Lung |
| Kidney Antibodies | >93 publications with PP2Ac and Kidney |
| Vascular Antibodies | >87 publications with PP2Ac and Vascular |
| Eye Antibodies | >40 publications with PP2Ac and Eye |