Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7]
Referência TA500641
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Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7]
SKU
TA500641
Anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7)
| Product Data | |
| Clone Name | OTI6C7 |
|---|---|
| Application | IF, IHC, Simple Western, WB |
| Recommended Dilution | WB 1:2000, IHC 1:50, IF 1:100; Simple Western 1:20-1:50 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 43.0 kDa |
| Gene Name | protein kinase cAMP-dependent type I regulatory subunit alpha |
| Database Link | |
| Background | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. |
| Synonyms | ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; TSE1 |
| Reference Data | |
| Protein Categories | Endocrine and metabolic diseases, Intracellular Proteins |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Apoptosis, Insulin signaling pathway |
| Product Manuals |
| FAQs |
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| SDS |
| Antibody Resources |
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| SKU | Description | Size | ||
|---|---|---|---|---|
| CF500641 | Carrier-free (BSA/glycerol-free) PRKAR1A mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) | 100 ug | | |
| TA500641AM | PRKAR1A mouse monoclonal antibody, clone 6C7, Biotinylated | 100 ul | | |
| TA500641BM | PRKAR1A mouse monoclonal antibody, clone 6C7, HRP conjugated | 100 ul | | |
| TA500641S | Anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) | 30 ul | | |
Citations
Primary Intra‐abdominal Melanoma Arising in Association with Extracutaneous Blue Nevus: A Report of Two Cases
Shah, Folpe, Miller et al
Histopathology (2020)
Shah, Folpe, Miller et al
Histopathology (2020)
Cited by 6
AI Summary: In this study, the OriGene PRKAR1A antibody (clone OTI6C7) was used for immunohistochemical analysis to help characterize the molecular features of primary intra-abdominal melanoma associated with extracutaneous blue nevus.
Snippet: This article is protected by copyright. All rights reserved monoclonal YR145;1/200;Cell Marque), DOG1 (clone K9;1/100;Leica (Novocastra), PRKAR1A (clone OTI6C7;1/2000;Origene) and BAP1 (mouse monoclonal C-4 antibody;1:100 dilution;Santa Cruz ) using;- This article is protected by copyright. All rights reserved monoclonal YR145
Calcification and ossification in conventional schwannoma: A clinicopathologic study of 32 cases
Din, Fritchie, Tariq et al
Neuropathology (2019)
Din, Fritchie, Tariq et al
Neuropathology (2019)
Cited by 11
AI Summary: OriGene contributed to this study by providing the anti-PRKAR1A antibody (clone 6C7) used for immunohistochemical analysis of schwannoma tissue samples.
Snippet: … Immunohistochemistry was performed on 4-μm-thick sections of formalin-fixed, paraffinembedded available tissues including psammomatous calcifications, and the sections were processed with steam heat-induced epitope retrieval, using a Ventana Benchmark XT platform (Ventana Corp., Tucson, AZ, USA), Ultraview diaminobenzidine detection and commercially available antibodies to protein kinase cyclic adenosine monophosphate-dependent type I regulatory subunit alpha (PRKAR1A) (clone 6C7, 1:8000;Origene, Rockville, MD, USA). …
Abstracts from USCAP 2019: Cardiovascular Pathology (299-316)
Lab Invest (2019) 99 (Suppl 1), 1-16 DOI: 10.1038/s41374-019-0211-3
Lab Invest (2019) 99 (Suppl 1), 1-16 DOI: 10.1038/s41374-019-0211-3
AI Summary: OriGene products, specifically the anti-TMEM106B antibody (Cat# TA803039), were used in this study to detect TMEM106B protein expression in cardiovascular tissue samples, aiding in the investigation of its role in cardiovascular pathology.
Fibrolamellar Carcinoma in the Carney Complex: PRKAR1A Loss Instead of the Classic DNAJB1-PRKACA Fusion
Rondell, Lackner, Terracciano et al
Hepatology (2017)
Rondell, Lackner, Terracciano et al
Hepatology (2017)
AI Summary: OriGene's PRKAR1A antibody (Clone OTi6C7) was used to assess PRKAR1A protein expression in tumor samples, helping demonstrate the loss of this regulatory protein in fibrolamellar carcinoma cases associated with the Carney complex.
Snippet: Clone OV-TL 12/30;Dako;CA), CD68 (1: 1500;Clone KP1;Dako, CA), Arginase (predilute;Clone SP156;Cell Marque, CA) and PRKAR1A (1:2000;Clone OTi6C7;Origene, MD). FISH Break apart FISH for rearrangement of the PRKACA locus was performed as
Hepatic adenomas with synchronous or metachronous fibrolamellar carcinomas: both are characterized by LFABP loss
Graham, Terracciano, Meves et al
Mod. Pathol. (2016)
Graham, Terracciano, Meves et al
Mod. Pathol. (2016)
Cited by 23
AI Summary: In this study, OriGene's PRKAR1A antibody (clone OTI6C7) was used for immunohistochemical analysis to help characterize protein expression in hepatic adenomas and fibrolamellar carcinomas.
Snippet: using antibodies to Arginase-1(predilute, clone SP156;Cell Marque), HepPar1 (predilute, clone OCH1E5;Ventana), cytokeratin 7 (1:100, Clone OV-TL 12/30;Dako), CD68 (clone KP1, 1:50-1:100;Dako), PRKAR1A (1:2000, clone OTI6C7;Origene Technologies), LFABP ... Cite SaveSaving
Defects of the Carney complex's gene (PRKAR1A) in odontogenic tumors
Sousa, Gomez, Diniz et al
Endocr. Relat. Cancer (2015)
Sousa, Gomez, Diniz et al
Endocr. Relat. Cancer (2015)
AI Summary: In this study, OriGene's mouse monoclonal antibody against PRKAR1A protein (clone 6C7) was used for immunohistochemical analysis to assess PRKAR1A protein expression in odontogenic tumors.
Snippet: knittel glass adhesive slides (Waldemar Knittel Glasbearbeitungs- GmbH, Braunschweig, 168 Germany). Immunohistochemical reactions were performed using a mouse monoclonal 169 antibody against PRKAR1A protein (clone 6C7) (OriGene, Rockville, MD, USA ... Cite SaveSaving
Showing 1-6 of 7 papers.
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