Prolactin Ovine Protein

Referência OPPA00505-5UG

Tamanho : 5µg

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Prolactin Ovine Protein (OPPA00505)
Product Info
Predicted Species ReactivitySheep
Product FormatLyophilized from concentrated solution with 0.02-0.03% NaHCO3
Additional InformationProtein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC DNAman computer analysis program of protein sequences.
Reconstitution and StorageIt is recommended to reconstitute the lyophilized Prolactin in sterile 18M-omega-cm H2O or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
PurificationThe Prolactin n is purified by proprietary chromatographic techniques.
Concentration1 mg/ml (prior to lyophilization)
PurityGreater than 99.0% as determined by a) Gel filtration analysis b) analysis by SDS-PAGE
Biological ActivityIs fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Peptide SequenceThe sequence of the first five N - terminal amino acids was determined and was found to be Ala - Thr - Pro - Val - Cys - Pro.
SourceE. coli
Gene SymbolProlactin
Alias SymbolsMammotropin, Luteotropic hormone, Luteotropin, PRL, Prolactin Ovine, Prolactin Ovine Recombinant
NCBI Gene Id443317
Protein NameProlactin
Description of TargetProlactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation. Prolactin Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids.
Uniprot IDP01240
Protein Accession #NP_001009306.1
Protein Size (# AA)Recombinant
Molecular Weight23 kDa