PRMT6, Chemiluminescent, BioAssay™ Kit (Protein Arginine N-Methyltransferase 6, Histone-Arginine N-Methyltransferase, HRMT1L6)

PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3.

Intended Use:
The PRMT6 Direct Activity BioAssay™ kit is designed to measure PRMT6 activity for screening and profiling applications.

Test Principle:
The key to the PRMT6 Direct Activity BioAssay™ Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt

Applications:
Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.

Kit Components:
Plate precoated with histone substrate, 1x96 wells
PRMT6 human recombinant enzyme, 1x20ug
20uM S-adenosylmethionine*, 1x250ul
Primary antibody 4, 1x100ul
Secondary HRP-labeled antibody 2, 1x10ul
4x HMT assay buffer 6, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A, (transparent bottle), 1x6ml
HRP chemiluminescent substrate B, (brown bottle), 1x6ml
*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the
inhibitors.

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.