PR/PGR (phospho-S294) polyclonal antibody

Product Name :
PR/PGR (phospho-S294) polyclonal antibody Background :
The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor, PR-A and PR-B, which are transcribed from distinct, estrogen inducible promoters within a single copy of the PR gene. The PR-A and PR-B proteins are 90 kDa and 118 kDa respectively; the first 164 amino acids of PR-B are absent in PR-A. Progesterone bound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand dependent transdominant repressor of steroid hormone receptor transcriptiona activity. An inhibitory domain (ID), which maps to the amino terminus of the receptor, exists within both PR isoforms. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PR/PGR (S190) polyclonal antibody detects endogenous levels of Progesterone Receptor protein only when phosphorylated at Ser190. Immunogen :
Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 294. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of p-PGR (S294) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins) Lane2:sp2/0 cell lysate treated with EGF(0.1ng/ML,15mins) Lane3:PC12 cell lysate treated with PMA(100nM,15mins)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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