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Porcine IL-6 ELISA Kit
Porcine IL-6 ELISA Kit
RayBio® Porcine (pig) IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Porcine (pig) |
| Sensitivity | 45 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 45 pg/ml - 10000 pg/ml |
| Specificity | The sandwich ELISA antibody pair detects Porcine IL-6. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P26893 |
| Gene Id | 100628202|399500 |
| Gene Symbols | IL6 |
| Protein Name / Synonyms | Interleukin-6 (IL-6) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Porcine IL-6 qIPCR ELISA Kit
As low as $749.00
RayPlex® Porcine IL-6 Bead Assay
As low as $240.00
Target Background
Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-α and IL-1, and activation of IL-1Rα and IL-10.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Porcine IL-6 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-6 (IL6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Metabolic Shifts Induced by Treatment with Statin Influences Circulating Concentrations of the Stress Hormone, Cortisol, but Has Different Effects on Selected Cytokines, Adipokines and Neuropeptides in Lean and Fat Lines of Young Pigs
- Species:
- Porcine
- Sample Type:
- Plasma (visceral); Tissue Lysate / Homogenate (epicardial adipose tissue)
- Year:
- 2025
- Journal Name:
- Metabolites
- PubMed ID:
- 41441039
Mitochondrial transplant after ischemia reperfusion promotes cellular salvage and improves lung function during ex-vivo lung perfusion
- Species:
- Porcine
- Sample Type:
- left ventricle
- Year:
- 2023
- Journal Name:
- Journal of Heart and Lung Transplantation
Highly pathogenic porcine reproductive and respiratory syndrome virus-induced inflammatory response in porcine pulmonary microvascular endothelial cells and effects of herbal ingredients on main inflammatory molecules
- Species:
- Pig
- Sample Type:
- porcine pulmonary microvascular endothelial cells (MVECs)
- Year:
- 2023
- Journal Name:
- International Immunopharmacology
Angiogenic gene characterization and vessel permeability of dermal microvascular endothelial cells isolated from burn hypertrophic scar
- Species:
- Pig
- Sample Type:
- Dermal microvascular endothelial cells (DMVECs)
- Year:
- 2022
- Journal Name:
- Scientific reports
Concentrations of selected immunological parameters in the serum and processing fluid of suckling piglets and the serum and colostrum of their mothers
- Species:
- Porcine
- Sample Type:
- Other Body Fluid (processing fluid, colostrum); Serum
- Year:
- 2024
- Journal Name:
- BMC Veterinary Research
- PubMed ID:
- 38702674
Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells
- Species:
- Porcine
- Sample Type:
- Conditioned Media (Perfieral Blood Cells)
- Year:
- 2020
- Journal Name:
- BMC complementary medicine and therapies
Adenosine A 2A agonist improves lung function during ex vivo lung perfusion.
- Species:
- Pig
- Sample Type:
- Tissue Lysate (lung)
- Year:
- 2011
- Journal Name:
- The Annals of thoracic surgery
Effects of Fruit By-product Extracts on Blood Characteristics, Antioxidant Activity, and Immune Response in Pigs.
- Species:
- Porcine
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Journal of animal science and technology
Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway
- Species:
- Porcine
- Sample Type:
- Conditioned Media (Intestinal Epithelial cells)
- Year:
- 2012
- Journal Name:
- Infection and Immunity
Effects of levan-type fructan supplementation on growth performance, digestibility, blood profile, fecal microbiota, and immune responses after lipopolysaccharide challenge in growing pigs.
- Species:
- Porcine
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 24045486
