This porcine IFABP ELISA kit is a sandwich immunoassay for the quantitative measurement of intestinal fatty acid binding protein (FABP2) in serum and plasma, supporting intestinal epithelial injury and gut barrier research.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine IFABP/FABP2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IFABP/FABP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine IFABP/FABP2. You can calculate the concentration of Porcine IFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.

Small volumes of IFABP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2501296 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IFABP/FABP2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFABP. The ELISA analytical biochemical technique of the MBS2501296 kit is based on IFABP antibody-IFABP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFABP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFABP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).