POLYMAG CRISPR 200 TRANSFECTION REAGENT

Referência PNC40200

Tamanho : 200µL

Marca : OZ Biosciences

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Telefone : +1 850 650 7790

  • PolyMag Neo
  • PolyMag Neo

PolyMag Neo

Magnetofection Transfection reagent - for a superior nucleic acid delivery in primary and hard-to-transfect cells

PolyMag Neo Magnetofection™ Reagent is specifically designed to achieve high transfection efficiency combined with superior transgene expression level. It is composed of magnetic nanoparticles coated with cationic molecules. PolyMag Neo is the ideal transfection reagent for a wide variety of cells.

  • High transgene expression
  • High transfection efficiency
  • Multipurpose (various types of nucleic acids)
  • Universal (primary cells and cell lines)
  • Simple, ready-to-use and rapid
  • Non toxic
  • Compatible with and without serum-containing culture media

PolyMag Neo can also be used for Genome Edition using the CRISPR-Cas9 system (see Application Note in Technical Resources). It is design for the cotransfection with 2 plasmids encoding guide RNA and Cas9 endonuclease.

Sizes

  • 100 µL: 100 transfections with 1µg of DNA
  • 200 µL: 200 transfections with 1µg of DNA
  • 1000 µL: 1000 transfections with 1µg of DNA
  • #KC30200 : Starting Kit with Super Magnetic plate + 100 µL of PolyMag Neo, PolyMag + CombiMag
  • #KC30296 : Starting Kit with plate 96 magnets + 100 µL of PolyMag Neo, PolyMag CombiMag
  • #KC30400: Super Starting Kit with Super Magnetic plate + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag
  • #KC30496 : Super Starting Kit with plate 96 magnets + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag

Storage: +4°C

Shipping conditions: Room temperature

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Laugier L. Clin Infect Dis. 2017 May 30. doi: 10.1093/cid/cix506. B Lymphocytes siRNA PolyMag Neo Mitkin NA. Biochim Biophys Acta. 2018 Jul 10. pii: S0925-4439(18)30248-5. Beas-2B DNA PolyMag Neo Gowda P. Cytokine . 2021 Jun;142:155496. doi: 10.1016/j.cyto.2021.155496. BHK-21 SF Virus PolyMag Neo Kurena B. J Virol Methods. 2017 Mar 14. pii: S0166-0934(17)30089-7. BMEC – Brain microvascular endothelial cells DNA PolyMag Neo Su Z. Pathogens. 2021 Oct 12;10(10):1307 CHO-K1 DNA PolyMag Neo Izumi C. Biophys J. 2011 Jun 8;100(11):2614-22. Chondrocytes DNA PolyMag Neo Megias J. Biochem Pharmacol. 2009 Jun 15;77(12):1806-13. COS-1 DNA PolyMag Neo Takeda K. J Clin Microbiol. 2013 Jul;51(7):2238-44. COS-7 DNA PolyMag Neo Deguchi M. J Clin Lab Anal. 2017 Oct 6. doi: 10.1002/jcla.22334. COS-7 DNA PolyMag Neo Takeda K. J Clin Microbiol. 2013 Jul;51(7):2238-44. Epithelial cells DNA PolyMag Neo Dutta RK. 2021 Biomed and Pharmacother. 10.1016/j.biopha.2021.112090 Epithelial Cells siRNA PolyMag Neo Ueta M. Brain Res Bull. 2010 Feb 15;81(2-3):219-28. Epithelial Cells siRNA PolyMag Neo Ueta M. Prog Retin Eye Res. 2012 Nov;31(6):551-75. HCT116 DNA PolyMag Neo Suemizu H. PLoS One. 2013 Dec 17;8(12):e82708. HEK-293 DNA PolyMag Neo Hinuma S. Vaccines (Basel) . 2022 Nov 30;10(12):2050. HEK-293 siRNA PolyMag Neo Choi S. Nucleic Acids Res . 2022 Jun 14;gkac509 HEK-293 siRNA PolyMag Neo Hinuma S. Viruses . 2022 Jan 7;14(1):105. HEK-293 siRNA PolyMag Neo Koide N. Biochim Biophys Acta Mol Cell Res . 2021 Jun;1868(7):119043. HEK-293 siRNA PolyMag Neo Hinuma S. Viruses. 2021 Jul; 13(7): 1334. HEK-293 siRNA PolyMag Neo Fujita K. Biochim Biophys Acta Mol Cell Res. . 2020 Sep 18;1868(1):118859. HEK-293 DNA PolyMag Neo Fujita K. Biochim Biophys Acta Mol Cell Res. . 2020 Sep 18;1868(1):118859. HepG2 DNA PolyMag Neo Hinuma S. Vaccines (Basel) . 2022 Nov 30;10(12):2050. HK2 DNA PolyMag Neo Rayego-Mateos S. J Am Soc Nephrol. 2020 Jul 6;ASN.2019111206. HUVEC DNA PolyMag Neo Cailleteau L. J Cell Sci. 2010 Jul 15;123(Pt 14):2491-501. MEF DNA PolyMag Neo Nogueira V. EMBO J. 2012 Mar 7;31(5):1134-46. MNT1 DNA PolyMag Neo Koroma DC. book chapter 2021 https://doi.org/10.1201/b22460 MNT1 DNA PolyMag Neo Koroma DC. bioRxiv 2021. doi.org/10.1101/2021.02.05.430016 MNT1 DNA PolyMag Neo Ozdeslik RN. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11508-11517. N2a DNA PolyMag Neo Chou CC. Nat Neurosci. 2018 Jan 8. doi: 10.1038/s41593-017-0047-3. Neural Crest Cells DNA PolyMag Neo Latta EJ. Mech Dev. 2012 Jan-Feb;128(11-12):560-76. Neuro-2a / N2a DNA PolyMag Neo Valdez-Sinon AN. Star Protocols 2020 doi.org/10.1016/j.xpro.2020.100083 Pollen DNA PolyMag Neo Issa JP. Patent 2020 20200354732 RAW 264.7 DNA Polymag Neo Umezu R. Arterioscler Thromb Vasc Biol. 2020 Jun 4;ATVBAHA120314383. Striatal cells DNA PolyMag Neo Marion S. J Biol Chem. 2014 Mar 11. Trabecular Meshwork DNA PolyMag Neo Tellios N. Sci Rep. 2017 Apr 11;7(1):812. TS/A SF Virus PolyMag Neo Kurena B. J Virol Methods. 2017 Mar 14. pii: S0166-0934(17)30089-7. U-2-OS siRNA PolyMag Neo Choi S. Nucleic Acids Res . 2022 Jun 14;gkac509 U-937 DNA PolyMag Neo Gowda P. Cytokine . 2021 Jun;142:155496. doi: 10.1016/j.cyto.2021.155496.

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Results Polymag Neo

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MSDS Polymag Neo

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Protocol Polymag Neo

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Application Note PolyMag Neo

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Magnetofection Brochure

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KPC40100
 100µL+amagneticplate