pMirTarget 3' UTR Assay Vector
Referência PS100062
Tamanho : 10ug
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pMirTarget 3' UTR Assay Vector
SKU
PS100062
pMirTarget, the cloning vector for all 3' UTR clones for miRNA target validation, 10ug
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Citations
In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
Mallén, Rotllan, Griñán et al
Am J Physiol Cell Physiol (2025)
Mallén, Rotllan, Griñán et al
Am J Physiol Cell Physiol (2025)
Cited by 1
AI Summary: OriGene products, specifically the pMirTarget control vector (Cat.#PS100062) and the pCCR7wt reporter construct containing the CCR7 3’UTR (Cat.#SC211596), were used to assess the regulation of CCR7 expression by miR-125b-5p in this study.
Snippet: e then transfected with 100 ng of329the following reporter constructs: control pMirTarget (Origene Technologies,330Cat.#PS100062, Rockville, MD, USA), pCCR7wt which included the 3’UTR of the331CCR7 mRNA (Origene Technologies, Cat.#SC211596, Rockville, MD, USA) and332pCCR7Δ in which the miR-125b predicted binding site on CCR7 had been dele
In vivo inhibition of miR-125b modulates monocyte trafficking through the CCR7 receptor and attenuates atherosclerosis
Mallén, Varela, Rotllan et al
medRxiv (2024)
Mallén, Varela, Rotllan et al
medRxiv (2024)
Snippet: pMirTarget reporter constructs (PS100062, Origene Technologies, Maryland, USA): the p_CCR7.WT plasmid with the wild type 3′UTR region of CCR7 gene (#SC211596;Origene pCCR7–3′UTR CCR7 (NM_001838) Human 3′UTR Clone) and p_CCR7.Mut with the mutated miR-125b predicted binding site from CCR7-3′UTR (#SC2211596_CW306881;Origene pCCR7–3′UTR CCR7 (NM_001838) alone as reference controls or together with 40 nM mirVana hsa-miR-125b-5p miRNa mimic (#4464066-MC10148;Thermofisher Scientifics, Massachusetts USA) using 0.3 μL TransIT-X2 (#6004 MirusBio, Wisconsin USA) according to the manufacturer’s protocol. …
ZFP36-mediated mRNA decay regulates metabolism
Cicchetto, Jacobson, Sunshine et al
Cell Rep (2023) 42 (5), 112411 DOI: 10.1016/j.celrep.2023.112411
Cicchetto, Jacobson, Sunshine et al
Cell Rep (2023) 42 (5), 112411 DOI: 10.1016/j.celrep.2023.112411
Cited by 43
AI Summary: In this study, OriGene products PCMV6-Kan/Neo-Mouse Eno2 (Cat# MC201508) and pMirTarget vector (Cat# PS100062) were used to investigate how ZFP36-mediated mRNA decay regulates metabolic enzyme expression and activity.
Snippet: Cellular metabolism is tightly regulated by growth factor signaling, which promotes metabolic rewiring to support growth and proliferation. While growth factor-induced Save;PCMV6-Kan/Neo-Mouse Eno2 Origene Cat# MC201508 pMirTarget vector Origene Cat# PS100062 CMV-ZFP36 This paper N/A Renilla Luciferase William Lowry (University of California, Los Angeles) Cinkornpumin et al., 2017
miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met
Huang, Huang, Chen et al
Biomedicines (2021) 9 (10) DOI: 10.3390/biomedicines9101371
Huang, Huang, Chen et al
Biomedicines (2021) 9 (10) DOI: 10.3390/biomedicines9101371
Cited by 12
AI Summary: OriGene products, specifically the pMirTarget vector and the mutant c-Met 3′UTR luciferase plasmid (cat. no. PS100062), were used to construct reporter assays that demonstrated c-Met as a direct target of miRNA-148a in this study.
Snippet: AAACACACGUGACU-3′) (cat. no. CW306397) was cloned into the pMirTarget vector to construct the wild-type (WT) c-Met 3′UTR plasmid or the mutant c-Met 3′UTR luciferase plasmid (cat. no. PS100062;OriGene Technologies, Rockville, MD, USA). Cells (1 × 105) were seeded into 24-well plates for 1 day and cultured until the cells reached 70–90% confluence. Subsequently, cells were transfected wit
Knockdown of lncRNA NUTM2A‑AS1 inhibits lung adenocarcinoma cell viability by regulating the miR‑590‑5p/METTL3 axis
Wang, Zha, Wang
Oncol Lett (2021) 22 (5), 798 DOI: 10.3892/ol.2021.13059
Wang, Zha, Wang
Oncol Lett (2021) 22 (5), 798 DOI: 10.3892/ol.2021.13059
Cited by 30
AI Summary: OriGene's pMIR-REPORT luciferase vector (cat. no. PS100062) was used to generate wild-type and mutant NUTM2A-AS1 3'UTR reporter constructs, enabling the study to confirm the direct interaction between NUTM2A-AS1 and miR-590-5p in lung adenocarcinoma cells.
Snippet: …no. PS100062;OriGene Technologies, Inc.) to generate the WT‑3'‑UTR NUTM2A‑AS1 and MUT‑3'‑UTR NUTM2A‑AS1 constructs, respectively. …
Anti-miR-518d-5p overcomes liver tumor cell death resistance through mitochondrial activity
Fernández-Tussy, Rodríguez-Agudo, Fernández-Ramos et al
Cell Death Dis (2021) 12 (6), 555 DOI: 10.1038/s41419-021-03827-0
Fernández-Tussy, Rodríguez-Agudo, Fernández-Ramos et al
Cell Death Dis (2021) 12 (6), 555 DOI: 10.1038/s41419-021-03827-0
Cited by 28
AI Summary: OriGene products, specifically the human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and the empty vector pMiRTarget (cat. number PS100062), were used in luciferase reporter assays to investigate the regulatory effects of miR-518d-5p on c-Jun expression in liver tumor cells.
Snippet: A minimum of three replicates was included per experiment. Luciferase reporter assays. Human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and empty vector pMiRTarget (cat. number PS100062) were purchased from OriGene (USA);Human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and empty vector pMiRTarget (cat. number PS100062) were purchased from OriGene (USA)
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