Pig interleukin 17,IL-17 ELISA Kit
Referência CSB-E08057p-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 96 | ||||
Range % | 91-103 | |||||
1:2 | Average % | 92 | ||||
Range % | 85-101 | |||||
1:4 | Average % | 87 | ||||
Range % | 80-94 | |||||
1:8 | Average % | 109 | ||||
Range % | 102-117 | |||||
The recovery of pig IL-17A/IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 102 | 94-108 | ||||
EDTA plasma (n=4) | 94 | 88-101 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 |
pg/ml | OD1 | OD2 | Average | Corrected | |
2000 | 3.568 | 3.621 | 3.595 | 3.523 | |
1000 | 1.834 | 1.849 | 1.842 | 1.770 | |
500 | 1.149 | 1.092 | 1.120 | 1.049 | |
250 | 0.703 | 0.702 | 0.703 | 0.631 | |
125 | 0.374 | 0.356 | 0.365 | 0.293 | |
62.5 | 0.235 | 0.248 | 0.241 | 0.170 | |
31.25 | 0.148 | 0.153 | 0.151 | 0.079 | |
0 | 0.070 | 0.073 | 0.072 |
| |
and FAQs
This Pig Interleukin 17A (IL-17A/IL-17) ELISA Kit was designed for the quantitative measurement of Pig IL17A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25pg/ml-2000pg/ml and the sensitivity is 15.625pg/ml.
Citations
- A Zha,Ecotoxicology and environmental safety,2023
- H Luo,/,2024
- J Peng,Biology,2022
- Y Zhao,Mediators of Inflammation,2020
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Target Background
(From Uniprot)
- At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, gammadelta T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development.
- Alterations in the IL-17 regulated pathway of porcine colon in response to dietary supplementation with seaweeds and yeast are reported.
- conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gammadeltaTCR(+).
- Cloning and expression of interleukin-17.
KEGG:
STRING:
UniGene: