Western Blot (Cell lysate)
Western blot analysis of C6, 3T3, Hela lysates with PGM1 recombinant monoclonal antibody, clone R08-9G3 (Cat # RAB04883).
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human PGM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PGM1.
Theoretical MW (kDa)
61
Reactivity
Human, Mouse, Rat
Form
Liquid
Preparation Method
Mammalian cell (CHO) expression system
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Immunofluorescence
Immunocytochemistry
Immunoprecipitation
Western Blot (Cell lysate)
Western blot analysis of C6, 3T3, Hela lysates with PGM1 recombinant monoclonal antibody, clone R08-9G3 (Cat # RAB04883). - Gene Info — PGM1
Entrez GeneID
5236Protein Accession#
P36871Gene Name
PGM1
Gene Alias
-
Gene Description
phosphoglucomutase 1
Omim ID
171900Gene Ontology
HyperlinkGene Summary
Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 [PubMed 8257433]).[supplied by OMIM
Other Designations
OTTHUMP00000010519|OTTHUMP00000046842
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