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Dispatch Time: About 3 working days
- Product Name
- PerCP-Cy5.5 Anti-Human CD47 Antibody(CC2C6D4)
- Catalogue No.
- PCP55-30259
- Form
- liquid
- Conjugation
- PerCP-Cy5.5
- Conjugation Information
- PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG1, κ
- Clone ID
- CC2C6D4
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- Cd47|IAP|Integrin-associated protein|Leukocyte surface antigen CD47 antibody
- UniProt ID
- Q08722
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Human peripheral blood lymphocytes are stained with PerCP-Cy5.5 Anti-Human CD47 Antibody (filled gray histogram). Unstained lymphocytes (black histogram) are used as control. - Background
- CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.