Manual| Component | CU101-01 | CU101-02 | CU101-03 |
| 2×Assembly Mix | 50 μl | 100 μl | 3×100 μl |
pEASY-Uni Seamless Cloning and Assembly Kit
Referência CU101-03
Tamanho : 60rxns
Marca : TransGen Biotech
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Product Details
This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25 bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of up to 15 fragments.
Features
• Fast: 5~15 minutes.
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Applications
Short and long fragment cloning, multi-fragment assembly, high-throughput cloning, etc.
Storage
at -18℃ or below for two years.
Shipping
Dry ice (-70℃)
Product Composition
References
1 Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)
2 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022(IF 14.90)
3 Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)
4 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)
5 Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 12.12)
6 Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)
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