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- Product Name
- PE-Cy5 Anti-Human CD43 Antibody(HI161)
- Catalogue No.
- PC5-30169
- Form
- liquid
- Conjugation
- PE-Cy5
- Conjugation Information
- PE-Cy5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG1, κ
- Clone ID
- HI161
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- Leukocyte Sialoglycoprotein|Leukosialin|Ly-48|Sialophorin antibody
- UniProt ID
- P16150
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Staining of normal human peripheral blood cells with FITC Anti-Human CD3 Antibody and PE-Cy5 Anti-Human CD43 Antibody(HI161) (left) or PE-Cy5 Mouse IgG1, κ Isotype Control (right). Cells in the lymphocytes gate were used for analysis. - Background
- CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.