Specifications

Product Data
C-Tag	HA-His
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Expression Promoter	CMV
Features	ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal HA-His tags. Such clones are the best for detection and purification of the transgene expression using anti-HA or anti-His antibodies or a nickel affinity columns If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal HA-His-tagging vector, PS100017. V2_F Primer can be used to sequence the target sequence after cloning. XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Ambient

Product Manuals

TrueORF and PrecisionShuttle cloning vectors Application Guide

FAQs

FAQ for Tagged Cloning Vectors

SDS for PS100008

Vector Resources

Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety