Immunocytochemistry
Immunocytochemical staining of Hela with NFKB2 recombinant monoclonal antibody, clone R04-4D6 (Cat # RAB02063). (A) NFKB2 (green) and (B) DAPI (blue).
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with NFKB2 recombinant monoclonal antibody, clone R04-4D6 (Cat # RAB02063).
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human NFKB2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human NFKB2.
Theoretical MW (kDa)
Calculated MW: 97 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Preparation Method
Mammalian cell (CHO) expression system
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA).
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunofluorescence
Immunocytochemistry
Immunocytochemical staining of Hela with NFKB2 recombinant monoclonal antibody, clone R04-4D6 (Cat # RAB02063). (A) NFKB2 (green) and (B) DAPI (blue).Immunoprecipitation
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with NFKB2 recombinant monoclonal antibody, clone R04-4D6 (Cat # RAB02063). - Gene Info — NFKB2
Entrez GeneID
4791Protein Accession#
Q00653Gene Name
NFKB2
Gene Alias
LYT-10, LYT10
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Omim ID
164012Gene Ontology
HyperlinkGene Summary
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM
Other Designations
OTTHUMP00000020371|OTTHUMP00000059136|nuclear factor of kappa light chain gene enhancer in B-cells 2|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
- Interactomes
- Pathways
- Diseases