Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]
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Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]
SKU
SM1475P
Myeloperoxidase (MPO) mouse monoclonal antibody, clone 2C7, Purified
| Product Data | |
| Clone Name | 2C7 |
|---|---|
| Application | ELISA, FC, IHC, WB |
| Recommended Dilution | ELISA. Western blotting (Non Reducing Conditions, See Audrian et al. in Ref.1). Flow Cytometry: 1/50-1/100 (Membrane permeabilisation is required). The antibody clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by Flow Cytometry following cell permeabilisation. Immunohistochemistry on Frozen Sections: 1/1000-1/5000. Immunohistochemistry on Paraffin Sections: 1/500-1/1000. Recommended Positive Control: Bone Marrow Tissue. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Human Myeloperoxidase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8653 myeloma cell line. |
| Specificity | This antibody is specific to Myeloperoxidase (MPO). Recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. It also recognizes recombinant MPO in Western blots and weakly in ELISA. Negative Species: Rat. |
| Buffer | PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | myeloperoxidase |
| Database Link | |
| Background | Myeloperoxidase/MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kD) and two light chains (15 kD), the heavy chains being linked by a disulphide bond. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase auto-antibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. |
| Synonyms | MPO |
| Reference Data | |
| Protein Categories | Immune system diseases, Intracellular Proteins |
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Citations
Clonal hematopoiesis in patients with ANCA-associated vasculitis
Arends, Weiss, Christen et al
Haematologica (2019)
Arends, Weiss, Christen et al
Haematologica (2019)
AI Summary: Based on the provided snippet, OriGene products were not mentioned or used in the referenced paper "Clonal hematopoiesis in patients with ANCA-associated vasculitis."
Snippet: antibodies: either isotype control (clone 11711) from R&D Systems, anti-PR3 (clone 12.8) from Sanqui (Amsterdam, The Netherlands), or anti-MPO (clone 2C7) from Acris Antibodies GmbH (Herford, Germany) in a total assay volume of 100 μl in polypropylene tubes View as HTML
Primed PMNs in healthy mouse and human circulation are first responders during acute inflammation
Fine, Barzilay, Sun et al
Blood Adv (2019) 3 (10), 1622-1637 DOI: 10.1182/bloodadvances.2018030585
Fine, Barzilay, Sun et al
Blood Adv (2019) 3 (10), 1622-1637 DOI: 10.1182/bloodadvances.2018030585
AI Summary: OriGene’s anti-myeloperoxidase (MPO) antibody (clone 2C7) was used in this study to help identify and characterize neutrophil populations involved in acute inflammation.
Snippet: he following anti-mouse antibodies were used (see supplemental Table 1): Ly6G (1A8), F4/80 (BM8), CD11b (M1/70), CD66a (CC1), and CD63 (MVG-2). α-Citrullinated histone 3 (α-H3Cit) (polyclonal;Abcam), elastase (polyclonal;Abcam), myeloperoxidase (MPO) (2C7;OriGene), and goat anti-rabbit immunoglobulin G H&L, AF488 (Abcam) were also used
Lactoferrin Suppresses Neutrophil Extracellular Traps Release in Inflammation
Okubo, Kamiya, Urano et al
EBioMedicine (2016) 10, 204-15 DOI: 10.1016/j.ebiom.2016.07.012
Okubo, Kamiya, Urano et al
EBioMedicine (2016) 10, 204-15 DOI: 10.1016/j.ebiom.2016.07.012
Cited by 171
AI Summary: In this study, OriGene's anti-lactoferrin antibody (Cat# TA503595) was used to detect lactoferrin localization, helping to demonstrate that lactoferrin suppresses neutrophil extracellular traps (NETs) release during inflammation.
Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies
O'Brien, Abdulahad, Rutgers et al
Sci Rep (2015) 5, 11888 DOI: 10.1038/srep11888
O'Brien, Abdulahad, Rutgers et al
Sci Rep (2015) 5, 11888 DOI: 10.1038/srep11888
Cited by 55
AI Summary: In this study, OriGene products—specifically the PR3 (OriGene, TA500043) and MPO (OriGene, TA500054) antibodies—were used to detect and analyze the surface expression of ANCA autoantigens on monocyte subsets, helping to elucidate their role in ANCA vasculitis.
C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis
Schreiber, Xiao, Jennette et al
J Am Soc Nephrol (2009) 20 (2), 289-98 DOI: 10.1681/ASN.2008050497
Schreiber, Xiao, Jennette et al
J Am Soc Nephrol (2009) 20 (2), 289-98 DOI: 10.1681/ASN.2008050497
AI Summary: In this study, OriGene’s mouse C5a receptor (C5aR) knockout bone marrow cells (catalog #SCM1027) were used to demonstrate that deficiency of C5aR in bone marrow-derived cells protects mice from ANCA-induced glomerulonephritis, highlighting the critical role of C5aR in disease pathogenesis.
Showing 1-5 of 5 papers.
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