Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit
Referência MBS269943-96
Tamanho : 96-Strip-Wells
Marca : MyBiosource
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Telefone : +1 850 650 7790
Mothers Against Decapentaplegic Homolog 3 (SMAD3) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS269943Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse Smad3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Mothers Against Decapentaplegic Homolog 3 (Smad3), ELISA Kit
Full Product Name
Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit
Product Gene Name
Smad3 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Smad3 concentration
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
25,722 Da
OMIM
gene 613795
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of Smad3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Smad3 purchase
MBS269943 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Smad3. The ELISA analytical biochemical technique of the MBS269943 kit is based on Smad3 antibody-Smad3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Smad3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Smad3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 3
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
UniProt Entry Name
SMAD3_HUMAN
NCBI Protein Information
mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3
NCBI Summary for Smad3
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Show more UniProt Comments for Smad3
SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: 15q22.33
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; plasma membrane; nuclear inner membrane; nucleus; cytosol; receptor complex
Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transforming growth factor beta receptor binding; transcription factor activity
Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of osteoblast differentiation; negative regulation of mitotic cell cycle; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; ureteric bud development; transport; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration
Disease: Loeys-dietz Syndrome 3
Show more Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: 15q22.33
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; plasma membrane; nuclear inner membrane; nucleus; cytosol; receptor complex
Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transforming growth factor beta receptor binding; transcription factor activity
Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of osteoblast differentiation; negative regulation of mitotic cell cycle; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; ureteric bud development; transport; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration
Disease: Loeys-dietz Syndrome 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with Smad3 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| AGE/RAGE Pathway antibodies | AGE/RAGE Pathway Diagram |
| Adherens Junction Pathway antibodies | Adherens Junction Pathway Diagram |
| Adherens Junction Pathway antibodies | Adherens Junction Pathway Diagram |
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| Alpha6-Beta4 Integrin Signaling Pathway antibodies | Alpha6-Beta4 Integrin Signaling Pathway Diagram |
| Androgen Receptor Signaling Pathway antibodies | Androgen Receptor Signaling Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Chagas Disease (American Trypanosomiasis) Pathway antibodies | Chagas Disease (American Trypanosomiasis) Pathway Diagram |
Diseases associated with Smad3 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Fibrosis Antibodies | >852 publications with Smad3 and Fibrosis |
| Inflammation Antibodies | >320 publications with Smad3 and Inflammation |
| Disease Models, Animal Antibodies | >310 publications with Smad3 and Disease Models, Animal |
| Liver Diseases Antibodies | >215 publications with Smad3 and Liver Diseases |
| Kidney Diseases Antibodies | >195 publications with Smad3 and Kidney Diseases |
| Cardiovascular Diseases Antibodies | >194 publications with Smad3 and Cardiovascular Diseases |
| Adenocarcinoma Antibodies | >151 publications with Smad3 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >120 publications with Smad3 and Breast Neoplasms |
| Liver Cirrhosis Antibodies | >118 publications with Smad3 and Liver Cirrhosis |
| Heart Diseases Antibodies | >112 publications with Smad3 and Heart Diseases |
Organs/Tissues associated with Smad3 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >565 publications with Smad3 and Muscle |
| Blood Antibodies | >396 publications with Smad3 and Blood |
| Bone Antibodies | >376 publications with Smad3 and Bone |
| Kidney Antibodies | >360 publications with Smad3 and Kidney |
| Lung Antibodies | >354 publications with Smad3 and Lung |
| Liver Antibodies | >330 publications with Smad3 and Liver |
| Vascular Antibodies | >277 publications with Smad3 and Vascular |
| Connective Tissue Antibodies | >275 publications with Smad3 and Connective Tissue |
| Skin Antibodies | >224 publications with Smad3 and Skin |
| Heart Antibodies | >188 publications with Smad3 and Heart |
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