Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit
Referência CSB-E14265m-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Abbreviation
CK 18-M30
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 μIU/mL-1000 μIU/mL
Sensitivity
3.9 μIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 96 | |
| Range % | 89-104 | ||
| 1:200 | Average % | 95 | |
| Range % | 90-100 | ||
| 1:400 | Average % | 92 | |
| Range % | 87-101 | ||
| 1:800 | Average % | 93 | |
| Range % | 90-97 |
Recovery
| The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 90-104 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Mouse CK 18-M30 ELISA Kit was designed for the quantitative measurement of Mouse CK 18-M30 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.
Citations
- E Gart,The FASEB Journal,2022
- I Pinheiro,Biomedicines,2022
- Suliman Al-Fayoumi.et al,J Exp Pharmacol.,2018
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