Mouse IgM, Human ads Antibody : BGAL
Referência OASB01466
Tamanho : 1.0mL
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
Goat Anti-Mouse IgM Antibody - BGAL Conjugated (OASB01466)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgM Antibody - BGAL Conjugated (OASB01466) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | b-gal |
| Application | IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with mouse IgM; purified by affinity chromatography on mouse IgM covalently linked to agarose; reacts with the heavy chain of mouse IgM; cross-adsorbed against mouse IgG and IgA / human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgM |
| Purification | Affinity chromatography on mouse IgM covalently linked to agarose. |
| Concentration | Lot specific |
| Specificity | IgM |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Weareye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested orinhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azidecontainingcompounds in running water before discarding to avoid accumulation of potentially explosive deposits in leador copper plumbing. |
| Dilution | Immunofluorescence: FLISA: ELISA: FITC and BIOT conjugates R-PE, APC, CY5, and R-PE/CY7 conjugates TRITC and TXRD conjugates AP conjugate HRP conjugate BGAL conjugate BIOT conjugate <= 1 ug/106 cells <= 0.1 ug/106 cells 1:100-1:400 1:2,000-1:4,000 1:4,000-1:8,000 1:500 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescent staining of IgM+ mouse B lymphocytes, Indirect immunostaining in conjunction with mouse IgM monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD), and Cyanine 5 (CY 5) conjugatesare supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The Beta- galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS/NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.5 mg in 1.0 mL or0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
