Mouse IgG2c, Human ads Antibody : Biotin
Referência OASB01583
Tamanho : 1.0mg
Marca : Aviva Systems Biology
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Goat Anti-Mouse IgG2c Antibody - Biotin Conjugated (OASB01583)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgG2c Antibody - Biotin Conjugated (OASB01583) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, ELISPOT, IHC-P, WB |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with mouse IgG2c; purified by affinity chromatography on mouse IgG2c covalently linked to agarose; reacts with the heavy chain of C57BL/6 mouse IgG2c; cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgG2c |
| Purification | Affinity chromatography on mouse IgG2c covalently linked to agarose. |
| Concentration | 0.5 mg/mL |
| Specificity | IgG2c |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA or FLISA to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | FLISA: ELISA: FITC conjugate AP conjugate HRP conjugate BIOT conjugate 1:200 – 1:400 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,004 |
| Application Info | Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8 C - Reagents are stable for the period shown on the label if stored as directed. |
| Cross Absorption | Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins. |