Mouse IgG2b, Human ads Antibody : Biotin
Referência OASB01623
Tamanho : 0.5mg
Marca : Aviva Systems Biology
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Goat Anti-Mouse IgG2b Antibody - Biotin Conjugated (OASB01623)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgG2b Antibody - Biotin Conjugated (OASB01623) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, ICC |
| Additional Information | Description: Pepsin digest of Goat Anti-Mouse IgG2b, Human ads - Pooled antisera from goats hyperimmunized with mouse IgG2b; purified by affinity chromatography on mouse IgG2b covalently linked to agarose; reacts with the heavy chain of mouse IgG2b; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgG2b |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | 0.5 mg/mL |
| Specificity | IgG2b |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells 1:5,000-1:10,000 |
| Application Info | Indirect immunofluorescent staining in conjunction with mouse IgG2b monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
