Mouse CD8a/Ly-2 Antibody : APC/CY7
Referência OASB00301
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Mouse CD8a/Ly-2 Antibody : APC/CY7 (OASB00301)
| Datasheets/Manuals | Printable datasheet for Mouse CD8a/Ly-2 Antibody : APC/CY7 (OASB00301) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 53-6.7 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | APC-Cyanine 7 |
| Application | FC |
| Additional Information | Description: In the mouse, CD8 exists in two forms - (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha) and a beta chain (CD8 beta); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8+ beta- T cells mature extrathymically, while development of the CD8 alpha+ beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Spleen cells or thymocyte membranes |
| Concentration | 0.1 mg/mL |
| Specificity | CD8a |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts ofwater. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidicconditions. Dilute azide- containing compounds in running water before discarding to avoid accumulation of potentiallyexplosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody and Fluorescein and Biotin conjugates <= 2 ug/106 cells AF488 conjugate <= 1 ug/106 cells R-phycoerythrin-Texas Red conjugate <= 0.2 ug/106 cells R-phycoerythrin and Allophycocyanin conjugates <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells Cyanine 5 and AF647 conjugates <= 0.2 ug/106 cells R-PE-CY 5.5, R-PE-CY 7, APC-CY 5.5, and APC-CY 7 conjugates |
| Application Info | Flow cytometry 8, 9, Immunohistochemistry (acetone-fixed frozen sections) 10, Immunoprecipitation 1,4, In vivo and in vitro depletion of CD8Alpha+ cells 1 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 ug/106 cells Cells from BALB/c mesenteric lymph nodes were doublestained with anti-mouse CD8α-FITC and anti-mouse CD4-R-PE , gated on small lymphocytes and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA) |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PECY 5.5), allophycocyanin- Cyanine 5.5 conjugate (APC- CY 5.5), allophycocyanin- Cyanine 7 (APC- CY 7) andR- phycoerythrin- Texas Red (R- PE- TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL ofPBS/NaN3. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Ledbetter, J. A. and W. E. Seaman. 1982. Immunol. Rev. 68:197 2. Lefrancois, L. 1991. J. Immunol. 147:1746 3. Lefrancois, L. 1991. Immunology Today 12:436 4. Takahasi, K., M. Nakata, T. Tanaka, H. Adachi, H. Nakauchi, H. Yagita, and K. Okumura. 1992. Proc. Natl. Acad. Sci. USA. 89:5557 5. Hathcock, K.S. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 3.4.1 6. Kruisbeek, A. M. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1 7. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118 8. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63 9. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280 10. Taylor,J.L., D.J. Ordway, J. Troudt, M. Gonzalez- Juarrero, R.J. Basaraba, and I.M. Orme. 2005. Infect. Immun 73(8):5189. |
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| Gene Symbol | CD8A |
| Gene Full Name | T-cell surface glycoprotein CD8 alpha chain |
| Alias Symbols | Ly-, Ly-2, Ly-3, Ly-B, Lyt-, Ly-35, Lyt-2, BB154331 |
| NCBI Gene Id | 12525 |
| Protein Name | T-cell surface glycoprotein CD8 alpha chain |
| Uniprot ID | P01731 |
| Protein Accession # | NP_001074579.1 |
| Nucleotide Accession # | NM_001081110.2 |
