Mouse CD62L/L-selectin Antibody : APC
Referência OASB00502
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Mouse CD62L/L-selectin Antibody : APC (OASB00502)
| Datasheets/Manuals | Printable datasheet for Mouse CD62L/L-selectin Antibody : APC (OASB00502) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | MEL-14 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | C3H/eb cloned mouse B cell lymphoma 38C-13 |
| Concentration | 0.1 mg/mL |
| Specificity | CD62L |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water.If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions.Dilute azide- containing compounds in running water before discarding to avoid accumulation of potentially explosivedeposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 2 ug/106 cells Biotin conjugate <= 2 ug/106 cells R-phycoerythrin conjugate <= 0.1 ug/106 cells Allophycocyanin and Allophycocyanin-Cyanine 7 conjugates <= 0.1 ug/106 cells Spectral Red conjugate <= 0.1 ug/106 cells R-phycoerythrin-Cyanine 7 conjugate <= 0.1 ug/106 cells |
| Application Info | Flow cytometry 1-14, Immunohistochemistry (frozen sections) 14, Immunoprecipitation 2, 7, In vivo and in vitro blocking of adhesion 1, 2, 7-9, Cytotoxicity studies 6 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution for the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 0.03 Ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse CD62L-R-PE and rat anti-mouse CD3έ-FITC. Lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg ofpurified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine- containingbuffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), and allophycocyanin- Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do notfreeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 C immediatelyupon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Gallatin, W.M., I.L. Weissman, and E.C. Butcher. 1983. Nature 304:30 2. Siegelman, M.H., I.C. Cheng,, I.L. Weissman, and E.K. Wakeland. 1990. Cell 61:611 3. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3521 4. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3529 5. Reichert, R.A., L. Jerabek, W.M. Gallatin, E.C. Butcher, and I.L. Weissman. 1986. J. Immunol. 136:3535 6. Iwabuchi, K., J. Ohgama, K. Ogasawara, C. Iwabuchi, I. Negishi, R.A. Good, and K. Onoe. 1991. Immunobiology 182:161 7. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313 8. Pizcueta, P., and F.W. Luscinskas. 1994. Am. J. Pathol. 145:461 9. Ley, K., D.C. Bullard, M.L. Arbones, R. Bosse, D. Vestweber, T.F. Tedder, and A.L. Beaudet. 1995. J. Exp. Med. 181:669 10. Jung, T.M., W.M. Gallatin, I.L. Weissman, and M.O. Dailey. 1988. J. Immunol. 141:4110 11. Kishimoto, T.K., M.A. Jutila, E.L. Berg, and E.C. Butcher. 1989. Science 245:1238 12. Mobley, J.L., and M.O. Dailey. 1992. J. Immunol. 148:2348 13. Sprent, J., and D.F. Tough. 1994. Science 265:1395 14. Aviva Systems Biology, Inc. Unpublished observations. |
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| Gene Symbol | SELL |
| Gene Full Name | L-selectin |
| Alias Symbols | L, Lya, CD62, Lnhr, Ly-2, Lyam, CD62L, L-sel, LECAM, Ly-22, Ly-m2, Lyam1, Ly-m22, Lyam-1, LECAM-1, AI528707, L-selectin |
| NCBI Gene Id | 20343 |
| Protein Name | L-selectin |
| Uniprot ID | P18337 |
| Protein Accession # | NP_035476.1 |
| Nucleotide Accession # | NM_011346.2 |
