Mouse CD11b/Mac-1a Antibody : APC
Referência OASB00315
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Mouse CD11b/Mac-1a Antibody : APC (OASB00315)
| Datasheets/Manuals | Printable datasheet for Mouse CD11b/Mac-1a Antibody : APC (OASB00315) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 3A33 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD11b (integrin alphaM subunit) combines with CD18 (integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Peritoneal macrophages from B6D2 hybrid mice |
| Concentration | 0.1 mg/mL |
| Specificity | CD11b |
| Characterization | To insure acceptable performance, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative flow data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts ofwater. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid underacidic conditions. Dilute azide- containing compounds in running water before discarding to avoid accumulation ofpotentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate R-phycoerythrin-Cyanine 7 conjugate Allophycocyanin-Cyanine 7 conjugate Alexa Fluor 488 <= 1 ug/106 cells <= 1 ug/106 cells <= 1 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells |
| Application Info | Flow cytometry 1, Immunoprecipitation 1 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 ug/106 cells BALB/c splenocytes were incubated with rat anti-mouse CD11b and then stained with goat anti-rat IgG (H+L)-FITC. Large cells were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purifiedimmunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine- containing buffersalts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) , R- phycoerythrin- Cyanine 7 (R- PE- CY 7) and Allophycocyanin- Cyanine 7 (APCCY7)conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Donot freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Martin, A., R. Le Corre, P. Pellen,, D. Bourel, G. Merdrignac, B. Genetet, and L. Toujas. 1986. Tissue Antigens 28:15 2. Springer, T., G. Galfre, D.S. Secher, and C. Milstein. 1979. Eur. J. Immunol. 9:301 3. Springer, T.A., D. Davignon, M.- K. Ho, K. Kurzinger, E. Martz, and F. Sanchez- Madrid. 1982. Immunol. Rev. 68:171 4. Beller, D.I., T.A. Springer, R.D. Schreiber. 1982. J. Exp. Med. 156:1000 5. Sanchez- Madrid, F., P. Simon, S. Thompson, and T.A. Springer. 1983. J. Exp. Med. 158:586 6. Lub, M., Y. van Kooyk, and C.J. Figdor. 1996. J. Leukoc. Biol. 59:648 7. Kantor, A.B., A. M. Stall, S. Adams, L.A. Herzenberg, and L.A. Herzenberg. 1992. Proc. Natl. Acad. Sci. USA 89:3320 8. Ault, K.A., and T.A. Springer. 1981. J. Immunol. 126:359 9. Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shortman. 1992. J. Exp. Med. 176:47 10. Leenen, P.J. M., M.F.T.R. de Bruijn, J.S.A. Voerman, P.A. Campbell, and W. van Ewijk. 1994. J. Immunol. Meth. 174:5 11. Gresham, H.D., et al. 1991. J. Clin. Invest. 88:588 12. Springer, T.A. 1994. Cell 76:310. |
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| Gene Symbol | ITGAM |
| Gene Full Name | Integrin alpha-M |
| Alias Symbols | CR, CR3, CR3A, Ly-4, MAC1, Mac-, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik |
| NCBI Gene Id | 16409 |
| Protein Name | integrin alpha-M |
| Uniprot ID | P05555 |
