Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> Vimentin (FLJ36605, VIM)

Vimentin (FLJ36605, VIM)

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Referência V2122-01-500ul

Tamanho : 500ul

Marca : US Biological

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V2122-01 Vimentin (FLJ36605, VIM)

Clone Type
Monoclonal
Host
mouse
Source
porcine
Isotype
IgG1,k
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Bo Ca Ch Eq Fe Hm Hu Mk Mo Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:50 for 20 minute at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Sarcomas

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 0.N.602|Host: mouse|Source: porcine|Concentration: ~0.2mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |Purity: Purified by Protein G affinity chromatography from ascites.|Immunogen: Purified Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic|Specificity: Recognizes porcine Vimetin, a 57-60kD protein. Species Crossreactivity: human, monkey, bovine, equine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes porcine Vimetin, a 57-60kD protein. Species Crossreactivity: human, monkey, bovine, equine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.
References
1. Uusitalo, M., et al., Investigative Ophthalmology and Visual Science, 1995 Dec., 36(13): 2584-2591. 2. Abeln, E.C., et al., British Journal of Cancer, 1994 Aug., 70(2): 255-262.